A Simple, Robust and Handheld Sample Preparation Technique for Quantifying Canthaxanthin in Chicken Liver, Fat and Egg Yolk | Chapter 13 | Innovations in Biological Science Vol. 3

The present chapter described a fast, simple, and small-scale method of sample preparation followed by HPLC coupled with a photodiode array detector (PDAD) for quantification of canthaxanthin in chicken liver and fat, egg yolk. The permitted Canthaxanthin (CX) is a red pigment and is commonly added to feeds for chickens in order to achieve the desired chicken product color. The HPLC-PDAD was performed on a C18 column with an isocratic mobile phase. The analyte was extracted from the sample using a handheld ultrasonic homogenizer and purified by MonoSpin®-SI, a centrifugal monolithic silica spin mini-columns, and quantified < 15 min. The system-suitability evaluation is an essential parameter of HPLC determination, and it ascertains the strictness of the system used. The suitability was evaluated as the relative standard deviations of peak areas and retention times calculated for 20 replicate injections of a spiked egg yolk sample. The proposed method obtained average recoveries for the analyte analyte in the range of 93.5 - 101.0% with relative standard deviations ≤ 2.7%. The Limits of quantification in the liver, fat, and egg yolk were 0.48, 0.47, and 0.5 µg/g, respectively. The present procedure provided an easy-to-use, rapid, and space-saving and resulted in high recovery and repeatability with considerable saving of analysis time/cost. The procedure may be proposed as an international harmonized method for determining CX in domestic/imported chicken products.

Author(s) Details:

Naoto Furusawa,
Graduate School of Human Life Science, Osaka Metropolitan University, Osaka 558-8585, Japan.

Please see the link here: https://stm.bookpi.org/IBS-V3/article/view/14331

Water Mobile Phase HPLC Determination of Acetamiprid and Its Metabolite, IM-2-1 | Chapter 7 | Challenges and Advances in Chemical Science Vol. 9

Using an isocratic 100 percent water mobile phase, this chapter describes a safe HPLC approach for identifying acetamiprid (ATP) and its metabolite, IM-2-1 (IM2). Water as the mobile phase and a photodiode-array detector were used in chromatographic separations using an Inertsil® WP300 C4 (PDA). The whole run time was about 7 minutes, with ATP and IM2 retention times of 5.75 and 6.49 minutes, respectively. The linearity and system appropriateness were both well within the accepted international standards. The detection limits for ATP and IM2 were 0.013 g/mL and 0.006 g/mL, respectively. A safe HPLC method for measuring ATP and IM2 concurrently was developed, and it could be used to quantify animal-derived diets in the future.

Author (s) Details

N. Furusawa
Graduate School of Human Life Science, Osaka Metropolitan University, Osaka 558-8585, Japan.


View Book :- https://stm.bookpi.org/CACS-V9/article/view/6453

Determination of Residual Acetamiprid and Imidacloprid in Unpolished Rice by Centrifugal Spin Column Clean-up and Water Mobile Phase HPLC-Diode Array | Chapter 06 | New Innovations in Chemistry and Biochemistry Vol. 6

 This chapter offers an appropriate analysis method for antimicrobial drug residual monitoring in food. Following a 100 percent water mobile phase high-performance liquid chromatography (HPLC) connected photo-diode array, a speedy, simple, and space-saving sample preparation procedure is used.A PDA was developed to measure acetamiprid (ATP) and imidacloprid (ICP) in unpolished rice.described in this article A handheld ultrasonic extractor was used to extract the analytes from the sample.cleansed by MonoSpin® C18-CX, a centrifugal monolithic silica spin mini-homogenizer with waterWithin 20 minutes, sample -1 was measured on a column. The precision, accuracy, and system suitability are all important factors.The method's approval requirements are well within the international norms.

Author (S) Details

Naoto Furusawa
Graduate School of Human Life Science, Osaka City University, Osaka, Japan.

View Book:- https://stm.bookpi.org/NICB-V6/article/view/5299

An Organic Solvent-free and Simple Procedure for Quantifying Tetracycline and 4-epi-tetracycline in Milk | Chapter 12 | Research Aspects in Agriculture and Veterinary Science Vol. 4

 This chapter offers a new quantification approach for an effective veterinary medicine (which is commonly used) that leads to improved food residue monitoring from the standpoint of assuring food safety. A quick, straightforward, small-scale sample preparation followed by an isocratic mobile phase HPLC for measuring tetracycline (TC) and its 4-epimer, 4-epi-tetracycline (4eTC) in cow's milk was disclosed in this paper under organic solvent-free circumstances. Only homogenization using a handheld ultrasonic homogenizer and deproteinizing aqueous solution, followed by filtration with a disposable unit, was possible for sample preparation. An Inertsil WP300 C4 and isocratic 100 percent aqueous mobile with photodiode array detector were utilised to determine and identify the analyte. The data from the method validation met the worldwide analytical method acceptance requirements. The time it took to analyse one sample was less than seven minutes. No organic solvents were employed at any point during the procedure. The current method has the potential to be offered as an international harmonised analytical method for routine TC and 4eTC residue monitoring in cow's milk.

Author(S) Details

Naoto Furusawa
Graduate School of Human Life Science, Osaka City University, Osaka 558-8585, Japan.

View Book:- https://stm.bookpi.org/RAAVS-V4/article/view/4854

Analytical Technique for Quantifying of Residual Acetamiprid and Imidacloprid in Wheat | Chapter 2 | Current Advances in Chemistry and Biochemistry Vol. 10

 This chapter explains how to use a 100 percent water mobile phase high-performance liquid chromatography (HPLC) coupled photo-diode array detector (PDA) to measure acetamiprid (ATP) and imidacloprid (ICP) in wheat, as well as a rapid, straightforward, and space-saving sample preparation technique. The analytes were removed from the sample with water using a handheld ultrasonic homogenizer, purified using MonoSpin® C18-CX, a centrifugal monolithic silica spin mini-column, and measured in under 20 minutes per sample. The precision, accuracy, and system suitability all meet or exceed the international method acceptance standards. For ATP and ICP residue monitoring in real wheat, the process could be approved as an international harmonised analytical method.

Author (S) Details

Naoto Furusawa
Graduate School of Human Life Science, Osaka City University, Osaka 558-8585, Japan.

View Book :- https://stm.bookpi.org/CACB-V10/article/view/2622