A Simple, Robust and Handheld Sample Preparation Technique for Quantifying Canthaxanthin in Chicken Liver, Fat and Egg Yolk | Chapter 13 | Innovations in Biological Science Vol. 3

The present chapter described a fast, simple, and small-scale method of sample preparation followed by HPLC coupled with a photodiode array detector (PDAD) for quantification of canthaxanthin in chicken liver and fat, egg yolk. The permitted Canthaxanthin (CX) is a red pigment and is commonly added to feeds for chickens in order to achieve the desired chicken product color. The HPLC-PDAD was performed on a C18 column with an isocratic mobile phase. The analyte was extracted from the sample using a handheld ultrasonic homogenizer and purified by MonoSpin®-SI, a centrifugal monolithic silica spin mini-columns, and quantified < 15 min. The system-suitability evaluation is an essential parameter of HPLC determination, and it ascertains the strictness of the system used. The suitability was evaluated as the relative standard deviations of peak areas and retention times calculated for 20 replicate injections of a spiked egg yolk sample. The proposed method obtained average recoveries for the analyte analyte in the range of 93.5 - 101.0% with relative standard deviations ≤ 2.7%. The Limits of quantification in the liver, fat, and egg yolk were 0.48, 0.47, and 0.5 µg/g, respectively. The present procedure provided an easy-to-use, rapid, and space-saving and resulted in high recovery and repeatability with considerable saving of analysis time/cost. The procedure may be proposed as an international harmonized method for determining CX in domestic/imported chicken products.

Author(s) Details:

Naoto Furusawa,
Graduate School of Human Life Science, Osaka Metropolitan University, Osaka 558-8585, Japan.

Please see the link here: https://stm.bookpi.org/IBS-V3/article/view/14331

Time-/Space-Saving Series of Operations for Quantifying Ractopamine in Beef under Organic Solvent-Free Conditions| Chapter 2 | Progress in Chemical Science Research Vol.3

 To forestall the dissemination of medication leftover food varieties, the powerful buildup scientific technique is key. This section portrayed a natural dissolvable free, straightforward, and time-/space-saving technique for test planning followed by elite execution fluid chromatography (HPLC) coupled photograph diode exhibit (PDA) identifier for measuring ractopamine (RP) in meat. The HPLC-PDA technique was utilized on an Inertsil®HILIC, hydrophilic connection chromatographic mode, segment with an isocratic watery versatile stage. A compact ultrasonic homogenizer was utilized to extricate an analyte from the example, which was then purged utilizing MonoSpin®SCX divergent solid silica turn smaller than usual segments and estimated in a short time. For successive examinations, a cluster of 24 examples could be broke down in roughly 7 h. The recommended approach accomplished normal recuperations for the anlyte in the scope of 95.2 - 103.1% with relative standard deviations ≤ 2.8%. As far as possible was 10 ng g-1 in the flank and liver. In the HPLC-PDA framework, RP was effectively distinguished in meat flank and liver examples by its maintenance time and assimilation range without the utilization of MS or MS/MS (very costly, stable/basic examination requires ability).

Author(s) Details:

Naoto Furusawa,
Graduate School of Human Life and Ecology, Osaka Metropplitan University, Osaka 558-8585, Japan.

Please see the link here: https://stm.bookpi.org/PCSR-V3/article/view/7957

Small-Scale and Time Shortening Sample Preparation Procedure to Quantify Astaxanthin, Canthaxanthin, and β-Apo-8’-Carotenoic Acid Ethyl Ester in Hen’s Egg Yolk | Chapter 6 | New Innovations in Chemistry and Biochemistry Vol.8

 Because eggs are highly nutritious, inexpensive, and readily available, they are a very important and basic food. It is critical to improve residual monitoring of eggs in order to maintain their safety. The current chapter described a simple, small-scale, and time-saving sample preparation procedure followed by simultaneous quantification of astaxanthin (AX), canthaxanthin (CX), and (beta)-apo-8'-carotenoic acid ethyl ester (ACAEE) in hen's egg yolk using a high-performance liquid chromatography (HPLC) coupled photodiode array (PDA) detector. The HPLC-PDA was performed on a C18 column with an isocratic mobile phase. Analytes were extracted from the sample with a handheld ultrasonic homogenizer in less than 30 minutes, purified with MonoSpin®-SI, a centrifugal monolithic silica spin mini-column, and quantified. For the three analytes, the proposed method provided average recoveries of 70.5-101.1 percent, with relative standard deviations of (le)5.0 percent. The quantitation limits for AX, CX, and ACAEE were 0.3 (mu)g g-1, 0.5 (mu)g g-1, and 1.0 (mu)g g-1, respectively. The examination of a single sample took about 30 minutes in total.

Author(s) Details:

N. Furusawa,
Graduate School of Human Life Science, Osaka Metropolitan University, Osaka, 558-8585, Japan.

Please see the link here: https://stm.bookpi.org/NICB-V8/article/view/6285 

Analytical Technique for Quantifying of Residual Acetamiprid and Imidacloprid in Wheat | Chapter 2 | Current Advances in Chemistry and Biochemistry Vol. 10

 This chapter explains how to use a 100 percent water mobile phase high-performance liquid chromatography (HPLC) coupled photo-diode array detector (PDA) to measure acetamiprid (ATP) and imidacloprid (ICP) in wheat, as well as a rapid, straightforward, and space-saving sample preparation technique. The analytes were removed from the sample with water using a handheld ultrasonic homogenizer, purified using MonoSpin® C18-CX, a centrifugal monolithic silica spin mini-column, and measured in under 20 minutes per sample. The precision, accuracy, and system suitability all meet or exceed the international method acceptance standards. For ATP and ICP residue monitoring in real wheat, the process could be approved as an international harmonised analytical method.

Author (S) Details

Naoto Furusawa
Graduate School of Human Life Science, Osaka City University, Osaka 558-8585, Japan.

View Book :- https://stm.bookpi.org/CACB-V10/article/view/2622