Escherichia coli O157:H7 is a pathotype of Enterohemorrhagic
Escherichia coli (EHEC) which is considered a public health problem
bacteria-induced food-borne diarrheas, bloody diarrhea and hemolytic uremic
syndrome that occurs at any age group. Three hundred stool samples were
obtained from children with bloody diarrhea, from January 2015 to May 2015,
their ages ranged from one month to five years who visited or were admitted as
"out-patients" to Al-Eskan paediatrics hospital, Al-Kadhumia
pediatric hospital or from a private clinic in Baghdad. Stool samples were cultured
in tetrathionate broth, then inoculated in MacConkey agar for isolation and
identification of lactose fermenter E. coli. Isolated E. coli is subcultured in
modified Sorbitol MacConkey Agar (SMAC-CT) for selection of E. coli O157:H7.
For confirmatory identification, the Latex agglutination test for E. coli
O157:H7 was used and PCR for the detection of Shiga toxin 1 (vt1) gene.
EHEC was found in 37 (12.33%) patients. The highest rate of 18 (48.64%) were in infants aged (3-12) months and 12 out of 37(32.43%) in the age group (13-24) months. 25 isolates were positive on Sorbitol MacConkey Agar with Cefixime and Tellurite (SMAC-CT). A commercial latex agglutination test revealed that E. coli: O157:H7 was found in 14(56%) out of 25 culture-positive cases while the other 11 (44%) isolates were E. coli O157: H- The results of PCR amplification of (vt1 gene) showed that 37 (12.33%) out of 300 stool samples were produced (130 pb) amplified band. All culture-positive samples (n=25) were also positive by PCR. These results concluded that this pathogen was considered one of the most important causes of gastrointestinal infection in children.
Author(s) Details:
Thanaa R. Abdulrahman,
Department of Microbiology, College of Medicine, Al-Nahrain
University, Iraq.
Please see the link here: https://stm.bookpi.org/IBS-V3/article/view/14242
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