The research has only yielded a partial comprehension of major depressive disorder (MDD) and the mechanisms underlying the antidepressant-like effects of Xiaoyaosan (XYS). Therefore, in this study, we aimed to explore the effects of XYS on chronic unpredictable mild stress- (CUMS-) induced changes in the neuronal and the astrocytic markers in the mouse hippocampus. The physical states and depressive-like behaviors in mice with CUMS were recorded. The serum contents of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) were measured. The protein and mRNA expressions and the immunoreactivities of glial fibrillary acidic protein (GFAP) and neuronal nuclei (NeuN) in mouse hippocampus were detected using a Western blot, qRT-PCR, and immunohistochemical staining, respectively. XYS treatment markedly improved the physical state and depressive-like behaviors in mice subjected to CUMS compared with the model group, and the serum contents of BDNF and GDNF were significantly upregulated. XYS treatment also elevated the protein and mRNA levels, as well as the immunoreactivity of GFAP in the hippocampus. However, CUMS did not influence NeuN expression. In conclusion, these results reveal that chronic administration of XYS elicits antidepressant-like effects in a mouse model of depression and may normalize glial fibrillary acidic protein expression in the hippocampi of mice with CUMS.
Author(s) Details:
Xiu-Fang Ding,
School of Basic Medical Science, Beijing University of Chinese
Medicine, Beijing-100029, China.
Yan Liu,
Henan
University of Chinese Medicine, Henan, China and School of Pre-Clinical
Medicine, Hubei University of Chinese Medicine, Wuhan, China.
Zhi-Yi Yan,
School of Basic Medical Science, Beijing University of Chinese
Medicine, Beijing-100029, China.
Xiao-Juan Li,
School of Basic Medical Science, Beijing University of Chinese
Medicine, Beijing-100029, China.
Qing-Yu Ma,
School of Basic Medical Science, Beijing University of Chinese
Medicine, Beijing-100029, China.
Zhong-Ye Jin,
School of
Basic Medical Science, Beijing University of Chinese Medicine, Beijing-100029,
China.
Yan Liu,
School of Basic Medical Science, Beijing University of Chinese
Medicine, Beijing-100029, China.
Yue-Hua Li,
Beijing Chaoyang Hospital, Capital Medical University, Beijing,
China.
Yue-Yun Liu,
School
of Basic Medical Science, Beijing University of Chinese Medicine,
Beijing-100029, China.
Zhe Xue,
School
of Basic Medical Science, Beijing University of Chinese Medicine,
Beijing-100029, China.
Jiaxu Chen,
School
of Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 11
North Third Ring Road Chaoyang District, Beijing-100029, China and
Formula-pattern Research Center, School of Traditional Chinese Medicine, Jinan
University, Guangzhou-510632, Guangdong, China.
Zhi-Ping Lv,
School of Traditional Chinese Medicine, Beijing
University of Chinese Medicine, No. 11 North Third Ring Road Chaoyang District,
Beijing-100029, China.
Please
see the link here: https://stm.bookpi.org/IPTDFTPX/article/view/11271
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