The aim of this study search out develop a alone isocratic phase HPLC method for the concurrent determination of Eprosartan mesylate and hydrochlorthiazide . The concurrent detection of hydrochlorthiazide and eprosartan mesylate together has been adept through the development and validation of a plain, fast, sensitive, and exact reverse phase souped up liquid chromatographic (RP-HPLC) method. Two together medications were separated by chromatography utilizing a Purospher BDS C18 column (250 mm × 4.6 mm id, 5µm atom size). The travelling phase involving of acetonitrile :methanol:0.01M KH2PO4 buffer (40:40:10) was brought at a flow rate of 1.0mL/min. The discovery was carried out at 270nm. The overall run period is 5 minutes, and the memory times for Eprosartan mesylate are 3.56 notes of meeting and 4.62 minutes, respectively. The assay for hydrochlorthiazide and eprosartan mesylate is uninterrupted within the aggregation range of 216-576µg/mL and 9-24µg/mL respectively. The judgments of the analysis have happened corroborated by recovery studies. The excipients in the formulations have no effect on the assay arrangement. The suggested approach was used to favorably detect Eprosartan mesylate and hydrochlorthiazide in drug formulations.
Author(s) Details:
Devika G. S.,
Department
of Pharmaceutical Analysis, Cherraan’s College of Pharmacy, 521, Siruvani Main
Road, Coimbatore-641039, Tamil Nadu, India.
Ramesh
Petchi R.,
Department
of Pharmacology, Cherraan’s College of Pharmacy, 521, Siruvani Main Road,
Coimbatore-641039, Tamil Nadu, India.
M. Sudhakar,
Department of Pharmaceutical Chemistry, Malla Reddy College of
Pharmacy, Maissamaguda, Dullapally, Secunderabad -14, India.
J. Venkateshwara Rao,
Department of Pharmaceutical Chemistry, Sultan Ul Uloom College of
Pharmacy, Road No3, Banjara Hills Secunderabad- 500034, India.
Please see the link here: https://stm.bookpi.org/ACPR-V3/article/view/12714
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