Molecular Identification of Hemoplasmas in Dogs Exposed to Ticks: A Retrospective Study| Chapter 2 | Newest Updates in Agriculture and Veterinary Science Vol. 2

 Canine hemotropic mycoplasmosis (hemoplasmosis) is a affliction associated with emptiness that has been stated in dogs with a extensive geographical distribution. The etiologic power is an epierythrocytic bacteria that is to say located in the erythrocytes` surface of the impressed host. There is a lack of information on hemoplasma contaminations in dogs in Mexico and particularly on the use of microscopic methods for the discovery of this microorganism. This study proposed to perform PCR assays and order analysis to consider dogs and ticks for the presence of dog hemoplasma species.The objective concerning this study was to recognize the presence of dog hemoplasmas in archived blood samples collected from 30 dogs and ticks composed from 18 of those dogs. The samples were processed for DNA origin and amplification by PCR accompanying 16S rRNA gene primers for Mycoplasma spp. Out of the samples resolved, the PCR assay allowed the labeling of DNA amplicons of the expected breadth in 20 and 4 of the blood and tick samples, respectively. Sequence study using the local adjustment bioinformatics tool (BLASTn) of 8 sequenced samples accompanied highly important identity percentages agreeable with the demeanor of various hemoplasma variety in the sequenced samples: Mycoplasma haemocanis (2 samples), Candidatus Mycoplasma haematoparvum (2 samples), Mycoplasma wenyonii (2 samples), Mycoplasma ovis (1 sample), and a nonculturable bacterium (1 sample). It is suggested to complete activity a more in-depth reasoning through the amplification and sequencing of additional structural genes present in the Mycoplasma spp genome, to prove whether all the recognized hemoplasma species can efficiently infect the dog, or the amplified domain of the 16S rRNA gene is not adequately variable between the different hemoplasmas that influence domestic mammals to allow bias of these Mycoplasma species.

Author(s) Details:

J. Juan Lira-Amaya,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

Grecia Martínez-García,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

Rebeca M. Santamaría-Espinosa,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

Carmen Rojas-Martínez,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

J. Antonio Álvarez-Martínez,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

J. Javier Pérez-de la Rosa,
CENAPA–SENASICA–SADER, Carretera Cuernavaca Cuautla No 8534, Jiutepec, Morelos, C.P. 62550, México.

Julio V. Figueroa-Millán,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

Please see the link here: https://stm.bookpi.org/NUAVS-V2/article/view/8920