Molecular Detection of Hemotropic Parasites in a Sheep Flock in Tabasco, Mexico: A Retrospective Study| Chapter 1 | Newest Updates in Agriculture and Veterinary Science Vol. 2

 The objective concerning this study was to recognize the possible appearance of tick-borne pathogens in ancestry samples and tick specimens that were obtained from few infested mammals in a sheep result unit in Tabasco, Mexico. To recognize the presence of pathogens communicated by ticks in sheep, a usefulness study was carried out at which point blood samples and tick samples were obtained, in 3 different periods, from 77 tick-diseased sheep situated in the Central region of united states of america of Tabasco, Mexico. Blood samples were processed for tiny and molecular labeling of Babesia spp, Anaplasma spp, and Mycoplasma spp. Morphological analysis of the composed ticks (including male and female larvae, nymphs, and men) allowed the labeling of Amblyomma maculatum, the Gulf Coast tick. Microscopic examination of blood smears displayed the absence of classic intraerythrocytic forms agreeable with Babesia spp. However, the occupancy of round intraerythrocytic inclusion corpses of approximately 1 μm in width, with a borderline arrangement and morphologically agreeable with Anaplasma sp, were observed in few samples analyzed, apart from small round frames attached to or next to ovine erythrocytes, suggesting the presence of Mycoplasma sp (earlier, Eperythrozoon sp). The use of genus-particular PCR molecular tests rooted the absence of Babesia sp in common people and allowed the identification of 7 samples (9%) beneficial for Anaplasma sp. The results obtained by PCR for Mycoplasma sp discovery showed that 62 (80.5%) of the samples proven were positive. DNA sequencing of amplicons got in the PCR test could take off light in this regard and delimit if the pathogen most feasible involved is Anaplasma marginale alternatively A. ovis as the sequence identity of the MSP-5 deoxyribonucleic acid is 99%. Similarly, DNA sequencing and BLASTn homology reasoning would show that the possible hemoplasma structure is either Mycoplasma wenyonii or Mycoplasma ovis as the sequence similarity of the rDNA gene is >95%, outside being able to illustrate the presence of a co-contamination in the sampled sheep. On the other hand, the act that A. maculatum may play in the transmission of pathogens in this place flock cannot be elucidated, as it is necessary to complete activity transmission experiments accompanying the different stages of A. maculatum to decide if this is done automatically or biologically.

Author(s) Details:

J. Juan Lira-Amaya,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

Nadia D. Ojeda-Robertos,
División Académica de Ciencias Agropecuarias, Universidad Juárez Autónoma de Tabasco, Carretera Vhsa-Teapa Km 25, Villahermosa, Tabasco CP 86290, México.

Francisco Martínez-Ibáñez,
CENAPA–SENASICA–SADER. Carretera Cuernavaca Cuautla No 8534, Jiutepec, Morelos, C.P. 62550, México.

J. Antonio Álvarez-Martínez,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

Carmen Rojas-Martínez,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

J. Javier Pérez-de la Rosa,
CENAPA–SENASICA–SADER. Carretera Cuernavaca Cuautla No 8534, Jiutepec, Morelos, C.P. 62550, México.

Grecia Martínez-García,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

Rebeca M. Santamaría-Espinosa,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.


Rebeca M. Santamaría-Espinosa,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

Please see the link here: https://stm.bookpi.org/NUAVS-V2/article/view/8919