Studies on Brown Midrib 6 and 12 Genes Introgression in Two Nigerien and One Malian Sorghum Varieties: A Practical Guide to Young Scientists with Limited Molecular Facility | Chapter 4 | Current Approaches in Science and Technology Research Vol. 12

 The introduction of bmr genes from a less well-adapted donor parent into well-adapted high-yielding biomass types with low nutritional value is critical for long-term livestock feeding in the Sahel. The major goal of this study was to introduce the bmr6 and bmr12 genes into Nigerien and Malian sorghum cultivars for grain and biomass production. As recurrent parents, the plant material included two enhanced sorghum types (Sepon82 and Kalla Kéné) and El mota, a farmer favourite variety. Redlan bmr6, Tx630 bmr12, and Wheatland bmr12 were the bmr donor parents. From January 2016 to June 2017, the hand emasculation technique was utilised to introduce bmr genes into recurrent parents to produce F3 and BC1F3 populations at the Sotuba research station in Mali. This strategy is still highly beneficial in the establishment of sorghum segregating populations via genetic introgression. During the population development, anthocyanin pigment and heterosis effects were important phenotypic features for identifying F1 and BC1F1 plants. In a cross between anthocyanin (purple plant) and tan plants, anthocyanin allowed the identification of F1 plants, whereas for both tan plants cross, The heterosis effect was crucial in distinguishing F1 from parental lines and determining successful crosses, as was bmr segregation in F2. The bmr segregation ration was analysed using the 2 test. The segregation ratios of bmr plants in F2 and BC1F2 demonstrated that a single recessive gene fit them well (3:1). In Niger during the 2017 cropping season, the bmr 6 and 12 genes were successfully transferred to three recurring parental varieties that are at the F4 and BC1F3 generation for grain and biomass yields potential tests.

Author (S) Details

Ousmane Seyni Diakité
Institut National de la Recherche Agronomique du Niger (INRAN), BP 429 Niamey, Niger.

Mamadou Aissata
Institut National de la Recherche Agronomique du Niger (INRAN), BP 429 Niamey, Niger.

Sissoko Aliou
Institut d’Economie Rurale (IER) BP 258 Rue Mohamed V. Bamako, Mali.

Sanogo Sekouba
Institut d’Economie Rurale (IER) BP 258 Rue Mohamed V. Bamako, Mali.

Mamoutou Kouressy
Institut d’Economie Rurale (IER) BP 258 Rue Mohamed V. Bamako, Mali.

Vaksmann Michel
CIRAD, UMR AGAP, BP 1813, Bamako, Mali and AGAP, Univ. Montpellier, CIRAD, INRA, Montpellier SupAgro, Montpellier, France.

Daniel K. Dzidzienyo
University of Ghana, West Africa Centre for Crop Improvement (WACCI). PMB LG 30, Legon Accra, Ghana.

Danquah Eric
University of Ghana, West Africa Centre for Crop Improvement (WACCI). PMB LG 30, Legon Accra, Ghana.

Tongoona Pangirayi
University of Ghana, West Africa Centre for Crop Improvement (WACCI). PMB LG 30, Legon Accra, Ghana.

Karim Traoré
Africa Rice Center, St. Louis, BP 96, Sénégal.

Niaba Teme
Institut d’Economie Rurale (IER) BP 258 Rue Mohamed V. Bamako, Mali.

View Book :- https://stm.bookpi.org/CASTR-V12/article/view/2596