Thrombosis is a great threat to human health that has
attracted increasing attention. Inflammation induces thrombosis, which further
exacerbates inflammatory development, thus representing a vicious cycle called
a thrombotic inflammatory response. This study established a murine thrombosis
model using κ-carrageenan to evaluate the inhibitory effects of Lactobacillus
delbrueckii subsp. bulgaricus KSFY07 (LDSB-KSFY07) on thrombus formation and
oxidative stress. Serum and liver tissue parameters, as well as intestinal
microbiota composition, were assessed through biochemical assays,
histopathological observation, quantitative polymerase chain reaction (qPCR),
and faecal microbial analysis. The results demonstrated that LDSB-KSFY07
significantly alleviated tail blackening in thrombotic mice, prolonged
activated partial thromboplastin time (APTT), and shortened thrombin time (TT),
while reducing levels of fibrinogen (FIB) and prothrombin time (PT).
Furthermore, LDSB-KSFY07 decreased malondialdehyde (MDA) content and enhanced
the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione
peroxidase (GSH-Px) in both serum and liver tissues of thrombotic mice.
Histopathological examination revealed that LDSB-KSFY07 ameliorated liver
tissue injury and reduced tail vein thrombus formation. At the molecular level,
LDSB-KSFY07 up-regulated the mRNA expression of copper/zinc‑SOD
(Cu/Zn‑SOD),
manganese‑SOD (Mn‑SOD), and GSH‑Px
in liver tissues. In contrast, it down-regulated the expression of NF‑κB p65, intercellular adhesion molecule‑1 (ICAM‑1),
vascular cell adhesion molecule‑1 (VCAM‑1), and
E-selectin in tail vein vascular tissues. Additionally, LDSB-KSFY07 increased
plasminogen activator inhibitor‑1 (PAI-1) mRNA expression and
decreased tissue plasminogen activator (tPA) expression in cardiac and tail
vein tissues. Faecal microbial analysis indicated that LDSB-KSFY07 modulated
the gut microbiota by increasing the abundance of Bacteroides, Lactobacillus,
and Bifidobacterium, while reducing the proportion of Firmicutes. In summary,
LDSB-KSFY07 exhibited notable antithrombotic and antioxidant effects in this
model, with higher concentrations showing efficacy comparable to that of
heparin. These findings suggest its potential as a probiotic candidate for
mitigating thrombosis and associated oxidative damage.
Author(s) Details
Pan Wang
Department of Traumatology, Chongqing University Central Hospital &
Chongqing Emergency Medical Center, Chongqing 400014, China.
Fang Tan
Chongqing Collaborative Innovation Center for Functional Food, Chongqing
Engineering Research Center of Functional Food, Chongqing Engineering
Laboratory for Research and Development of Functional Food, Chongqing
University of Education, Chongqing 400067, China.
Jianfei Mu
Chongqing Collaborative Innovation Center for Functional Food, Chongqing
Engineering Research Center of
Functional Food, Chongqing Engineering Laboratory for Research and Development
of Functional Food, Chongqing University of Education, Chongqing 400067, China.
Hongjiang Chen
Chongqing Collaborative Innovation Center for Functional Food, Chongqing
Engineering Research Center of Functional Food, Chongqing Engineering
Laboratory for Research and Development of Functional Food, Chongqing
University of Education, Chongqing 400067, China.
Xin Zhao
Chongqing Collaborative Innovation Center for Functional Food, Chongqing
Engineering Research Center of Functional Food, Chongqing Engineering
Laboratory for Research and Development of Functional Food, Chongqing
University of Education, Chongqing 400067, China.
Yanan Xu
Department of Traumatology, Chongqing University Central
Hospital & Chongqing Emergency Medical Center, Chongqing 400014, China.
Please see the book here :- https://doi.org/10.9734/bpi/mono/978-81-998509-9-6/CH5
No comments:
Post a Comment