Evaluating the Inhibitory Effect of Lactobacillus delbrueckii subsp. bulgaricus KSFY07 on Thrombosis | Chapter 5 | Application of Probiotics in Exercise and Thrombosis Inhibition

 

Thrombosis is a great threat to human health that has attracted increasing attention. Inflammation induces thrombosis, which further exacerbates inflammatory development, thus representing a vicious cycle called a thrombotic inflammatory response. This study established a murine thrombosis model using κ-carrageenan to evaluate the inhibitory effects of Lactobacillus delbrueckii subsp. bulgaricus KSFY07 (LDSB-KSFY07) on thrombus formation and oxidative stress. Serum and liver tissue parameters, as well as intestinal microbiota composition, were assessed through biochemical assays, histopathological observation, quantitative polymerase chain reaction (qPCR), and faecal microbial analysis. The results demonstrated that LDSB-KSFY07 significantly alleviated tail blackening in thrombotic mice, prolonged activated partial thromboplastin time (APTT), and shortened thrombin time (TT), while reducing levels of fibrinogen (FIB) and prothrombin time (PT). Furthermore, LDSB-KSFY07 decreased malondialdehyde (MDA) content and enhanced the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in both serum and liver tissues of thrombotic mice. Histopathological examination revealed that LDSB-KSFY07 ameliorated liver tissue injury and reduced tail vein thrombus formation. At the molecular level, LDSB-KSFY07 up-regulated the mRNA expression of copper/zinc‑SOD (Cu/Zn‑SOD), manganese‑SOD (Mn‑SOD), and GSH‑Px in liver tissues. In contrast, it down-regulated the expression of NF‑κB p65, intercellular adhesion molecule‑1 (ICAM‑1), vascular cell adhesion molecule‑1 (VCAM‑1), and E-selectin in tail vein vascular tissues. Additionally, LDSB-KSFY07 increased plasminogen activator inhibitor‑1 (PAI-1) mRNA expression and decreased tissue plasminogen activator (tPA) expression in cardiac and tail vein tissues. Faecal microbial analysis indicated that LDSB-KSFY07 modulated the gut microbiota by increasing the abundance of Bacteroides, Lactobacillus, and Bifidobacterium, while reducing the proportion of Firmicutes. In summary, LDSB-KSFY07 exhibited notable antithrombotic and antioxidant effects in this model, with higher concentrations showing efficacy comparable to that of heparin. These findings suggest its potential as a probiotic candidate for mitigating thrombosis and associated oxidative damage.

 

 

Author(s) Details

Pan Wang
Department of Traumatology, Chongqing University Central Hospital & Chongqing Emergency Medical Center, Chongqing 400014, China.

 

Fang Tan
Chongqing Collaborative Innovation Center for Functional Food, Chongqing Engineering Research Center of Functional Food, Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China.

 

Jianfei Mu
Chongqing Collaborative Innovation Center for Functional Food, Chongqing Engineering Research Center of Functional Food, Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China.

 

Hongjiang Chen
Chongqing Collaborative Innovation Center for Functional Food, Chongqing Engineering Research Center of Functional Food, Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China.

 

Xin Zhao
Chongqing Collaborative Innovation Center for Functional Food, Chongqing Engineering Research Center of Functional Food, Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China.

 

Yanan Xu

Department of Traumatology, Chongqing University Central Hospital & Chongqing Emergency Medical Center, Chongqing 400014, China.

 

Please see the book here :- https://doi.org/10.9734/bpi/mono/978-81-998509-9-6/CH5

 

Spinning, Oxidative Damage and Hemolysis in Athletes and Trained Middle-Aged Women| Chapter 1 | Current Overview on Disease and Health Research Vol. 10

 The purpose concerning this study search out demonstrate and ratify the link middle from two points hemolysis and oxidative damage in cases who practice spiraling containing middle-old women.Twelve healthful and concerning matter alive volunteers: 7 men, 40 ± 5 age traditional and 5 females, 40 ± 5 age traditional, prepared subjects, not busy in vying sports, were registered and studied. Measurement of malonyldialdehyde is usual as an sign of lipid peroxidation. The calculation of the hemolysis assays was done. The state of RBCs sheath were persistent through a conventional histological method outlined May-Grunwald from Merck (Germany).As regards the middle-old mothers thirty-four healthy mothers old 45 to 55 age were selected for this study. Lipid peroxidation was supposed by weighing thiobarbituric acid sensitive substances in antitoxin samples.The calm dossier show that there are meaningful distinctnesses not completely 60 min  subsequently preparation: in malonyldialdehyde worth (0.12 ± 0.05 nmol/ml) and in malonyldialdehyde value accompanying 2, 2’-azobis (2 amidinopropane) dihydrochloride (0.22 ± 0.05 nmol/ml), in hemolysis dossier until both 80 brief time period (oxidative lysis) and 120 brief time period (2, 2’ azobis (2-amidinopropane) dihydrochloride lysis) habitual by microscopical analysis.Immediately subsequently revolving a meaningful increase in TBARS (thiobarbituric acid reactive wealthes) (from 16.5 ± 2 to 25 ± 2 nmol MDA/mL antitoxin) was noticed (p < 0.05) compared to two together those motionless and control group (seldom exercised).The exercise (not completely subsequently 60 brief time period) applied to proven competitors, causes in ruling class intra-vascular hemolysis (120 brief time period hemolysis dossier).This process is guide in vivo alterations in RBC membranes similar to those in the direction of hereditary feeble diseases.We speculate these fundamental and perhaps also working alterations of the RBCs are result of an raised susceptibleness of the cells to material and/or synthetic stress alternatively compression of capillaries all along the extremity of an animate being seem the primary subscriber to exercise accompanying hemolysis.Finally, our dossier comparing prepared and lazy mothers allow us in the end that daughters committed in spinning exercise programs emphasize the benefits of balanced entertainment in this community.

Author(s) Details:

Giuseppe Gallo,
Laboratory of Cell Physiology, Department of Biology, Ecology and Earth Sciences, University of Calabria, Rende (CS) 87036, Italy.

Guglielmo Martino,
Laboratory of Cell Physiology, Department of Biology, Ecology and Earth Sciences, University of Calabria, Rende (CS) 87036, Italy.

Annarita Carino,
Laboratory of Cell Physiology, Department of Biology, Ecology and Earth Sciences, University of Calabria, Rende (CS) 87036, Italy.

Please see the link here: https://stm.bookpi.org/CODHR-V10/article/view/9391