Antifatigue and Antioxidant Effects of Lactobacillus fermentum HFY03 from Yak Yoghurt in a Mouse Model of Exercise-Induced Exhaustion |Chapter 2 | Application of Probiotics in Exercise and Thrombosis Inhibition

 

Yak yoghurt, a traditional, naturally fermented dairy product, is primarily produced in the Qinghai-Tibet Plateau. It harbours a rich diversity of microorganisms, including Lactobacillus fermentum (LF) HFY03, a lactic acid bacterium isolated from this source. The primary objective of this study was to investigate the effects of LF-HFY03 on anti-fatigue and antioxidant capacities in a mouse model of exercise-induced exhaustion. Mice were administered varying doses of LF-HFY03 via oral gavage for a duration of four weeks, with vitamin C serving as the positive control. This design allowed for the evaluation of the relationship between LF-HFY03 supplementation and the enhancement of antioxidant and anti-fatigue parameters in exhausted mice. The results demonstrated that both LF-HFY03 and vitamin C significantly prolonged the forced running time to exhaustion in mice. Notably, the high-dose LF-HFY03 group exhibited an effect more than threefold greater than that of the control group. A positive correlation was observed between the concentration of LF-HFY03 and the extension of exhaustion time. Furthermore, LF-HFY03 administration effectively reduced serum levels of urea nitrogen and lactic acid, while increasing concentrations of free fatty acids and hepatic glycogen. The activities of serum alanine aminotransferase (ALT), creatine kinase (CK), and aspartate aminotransferase (AST) were gradually decreased in response to LF-HFY03 supplementation. In terms of oxidative stress markers, LF-HFY03 dose-dependently reduced malondialdehyde (MDA) levels and elevated the activities of the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD). At the molecular level, LF-HFY03 upregulated the mRNA expression of CAT, copper/zinc-SOD (Cu/Zn-SOD), and manganese-SOD (Mn-SOD) in mouse liver tissue. Concurrently, in skeletal muscle, it enhanced the expression of the alanine/serine/cysteine/threonine transporter 1 (ASCT1) protein and the mRNAs of neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS), while downregulating the expression of tumour necrosis factor-alpha (TNF-α), syncytin-1, and inducible nitric oxide synthase (iNOS). In conclusion, these findings indicate that LF-HFY03 possesses significant anti-fatigue and antioxidant properties, highlighting its considerable potential for development and application as a probiotic-based nutritional supplement aimed at alleviating physical fatigue.

 

 

Author(s) Details

Junxiao Zhang
Chongqing Collaborative Innovation Center for Functional Food, Chongqing Engineering Research Center of Functional Food, Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China and School of Teacher Development, Chongqing University of Education, Chongqing 400067, China.

 

Ling Chen
Department of Pharmacy, Xindu District People’s Hospital of Chengdu, Chengdu, 610500 Sichuan, China.

 

Lingyan Zhang
School of Continuing Education, Chongqing University of Education, Chongqing 400067, China.

 

Qiuping Chen
Department of Education, Our Lady of Fatima University, Valenzuela 838, Philippines.

 

Fang Tan
Department of Public Health, Our Lady of Fatima University, 838 Valenzuela, Philippines.

 

Xin Zhao
Chongqing Collaborative Innovation Center for Functional Food, Chongqing Engineering Research Center of Functional Food, Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China and School of Teacher Development, Chongqing University of Education, Chongqing 400067, China.

 

Please see the book here :- https://doi.org/10.9734/bpi/mono/978-81-998509-9-6/CH2