Yak yoghurt, a traditional, naturally fermented dairy
product, is primarily produced in the Qinghai-Tibet Plateau. It harbours a rich
diversity of microorganisms, including Lactobacillus fermentum (LF) HFY03, a
lactic acid bacterium isolated from this source. The primary objective of this
study was to investigate the effects of LF-HFY03 on anti-fatigue and
antioxidant capacities in a mouse model of exercise-induced exhaustion. Mice
were administered varying doses of LF-HFY03 via oral gavage for a duration of
four weeks, with vitamin C serving as the positive control. This design allowed
for the evaluation of the relationship between LF-HFY03 supplementation and the
enhancement of antioxidant and anti-fatigue parameters in exhausted mice. The
results demonstrated that both LF-HFY03 and vitamin C significantly prolonged
the forced running time to exhaustion in mice. Notably, the high-dose LF-HFY03
group exhibited an effect more than threefold greater than that of the control
group. A positive correlation was observed between the concentration of
LF-HFY03 and the extension of exhaustion time. Furthermore, LF-HFY03
administration effectively reduced serum levels of urea nitrogen and lactic
acid, while increasing concentrations of free fatty acids and hepatic glycogen.
The activities of serum alanine aminotransferase (ALT), creatine kinase (CK),
and aspartate aminotransferase (AST) were gradually decreased in response to
LF-HFY03 supplementation. In terms of oxidative stress markers, LF-HFY03
dose-dependently reduced malondialdehyde (MDA) levels and elevated the
activities of the antioxidant enzymes catalase (CAT) and superoxide dismutase
(SOD). At the molecular level, LF-HFY03 upregulated the mRNA expression of CAT,
copper/zinc-SOD (Cu/Zn-SOD), and manganese-SOD (Mn-SOD) in mouse liver tissue.
Concurrently, in skeletal muscle, it enhanced the expression of the
alanine/serine/cysteine/threonine transporter 1 (ASCT1) protein and the mRNAs
of neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS),
while downregulating the expression of tumour necrosis factor-alpha (TNF-α),
syncytin-1, and inducible nitric oxide synthase (iNOS). In conclusion, these
findings indicate that LF-HFY03 possesses significant anti-fatigue and
antioxidant properties, highlighting its considerable potential for development
and application as a probiotic-based nutritional supplement aimed at
alleviating physical fatigue.
Author(s) Details
Junxiao Zhang
Chongqing Collaborative Innovation Center for Functional Food, Chongqing
Engineering Research Center of Functional Food, Chongqing Engineering
Laboratory for Research and Development of Functional Food, Chongqing
University of Education, Chongqing 400067, China and School of Teacher
Development, Chongqing University of Education, Chongqing 400067, China.
Ling Chen
Department of Pharmacy, Xindu District People’s Hospital of Chengdu,
Chengdu, 610500 Sichuan, China.
Lingyan Zhang
School of Continuing Education, Chongqing University of Education,
Chongqing 400067, China.
Qiuping Chen
Department of Education, Our Lady of Fatima University, Valenzuela 838,
Philippines.
Fang Tan
Department of Public Health, Our Lady of Fatima University, 838 Valenzuela,
Philippines.
Xin Zhao
Chongqing Collaborative Innovation Center for Functional Food, Chongqing
Engineering Research Center of Functional Food, Chongqing Engineering
Laboratory for Research and Development of Functional Food, Chongqing
University of Education, Chongqing 400067, China and School of Teacher
Development, Chongqing University of Education, Chongqing 400067, China.
Please see the book here :- https://doi.org/10.9734/bpi/mono/978-81-998509-9-6/CH2
