Objective: The present study aimed to isolate the conditional
amino acids present in the fresh leaf extracts of Alternanthera sessilis and
compare the isolated amino acids with amino acid standards.
Background: Primary metabolites usually perform intrinsic
functions and so present in all plants. Some common examples are carbohydrates,
ethanol, lipids, nucleic acid and proteins. Secondary metabolites are usually
not directly involved in growth, development or reproduction but are involved
in defense function.
Methods: The fresh plant material was used for extraction. For the
extracted sample solution, Paper chromatography and High-performance thin-layer
chromatography fingerprinting analysis were then carried out.
Results: Since Alternanthera sessilis is rich in protein, various
amino acids have been isolated in aggregates and Paper chromatography has been
performed. Amino acids are separated and identified using trial and error
method. Cysteine, glycine, aspartic acid and proline-like standard amino acids were
used for comparing with the isolated amino acids by High-performance thin layer
chromatography fingerprint analysis, giving an idea for the authentication of
the plant, its constituents and also provide parameters for quality control. The result obtained by qualitative evaluation
of HPTLC will be helpful in further quantitative estimation of each amino acid
and also for standardization of herbal formulations containing this plant.
Conclusion: The results scientifically validate the use of
Alternanthera sessilis in traditional medicine and also as a food. The present
study can be considered as a reference and can be used for validation of the
plant usage.
Author(s)
Details
Swarnakumari
S
Karpagam College of Pharmacy, Coimbatore, Tamil Nadu, India.
Mohan S
Karpagam College of Pharmacy, Coimbatore, Tamil Nadu, India.
Ida
Christi V E
Karpagam College of Pharmacy, Coimbatore, Tamil Nadu, India.
Abinaya
S
Karpagam College of Pharmacy, Coimbatore, Tamil Nadu, India.
Kalaivani
S
Karpagam College of Pharmacy, Coimbatore, Tamil Nadu, India.
Please see the book here:- https://doi.org/10.9734/bpi/prrat/v4/6650A
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