Hepatitis E virus (HEV) is the causative agent of hepatitis
E worldwide. The indispensability of the open reading frame 4 (ORF4) region in
the replication of HEV Genotype 1 (G1) has been demonstrated. Intrinsically
disordered protein (IDP) offers enormous potential as a druggable target.
Intrinsically disordered protein regions (IDPRs)/intrinsically disordered
proteins (IDPs) are characterized by a lack of defined tertiary structure under
physiological conditions. Given their prevalence in various diseases, IDPs are
attractive therapeutic targets. The ORF4 has shown its involvement in the
regulation of HEV due to the prevalence of intrinsically disordered regions
(IDRs) as IDPR or IDP. In this context, the current study explores the ORF4
protein as a drug target due to its intrinsic disorder (ID) protein
characteristic. Utilizing the homology modelling algorithm, the 3-dimensional
(3D) structures of the ORF4 target protein were designed and further assessed
through PROCHECK for the presence of clefts, tunnels and pores. Subsequently,
the ORF4 was examined for the overall fraction of intrinsic disorder content.
In this chapter, an overview of ORF4 has been provided in terms of its
structure-function relationship and contribution to several biological
processes through PPIs. The results propose that ORF4 protein could act as a
potential drug molecule, henceforth, accelerating the process of drug designing
strategies against HEV.
Author(s) Details
Zoya Shafat
Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia
Islamia, New Delhi 110025, India.
Please see the link:- https://doi.org/10.9734/bpi/mono/978-81-976932-1-2/CH5
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