Hepatitis E virus (HEV) is a small RNA virus. The unique
component open reading frame 4 (ORF4) has been demonstrated to perform crucial
functions in the HEV Genotype I (GT I). The amino acid pattern of HEV-ORF4 was
systematically examined by computer predictors to explicate its role in viral
pathogenesis. Amino acid distribution showed ORF4 was enriched with
disorder-promoting residues (Ala, Arg, Pro, Ser, Gly) and a few order-promoting
residues (Leu), in combination with structure-breaking resides (Gly and Pro).
The ORF4 showed a deficiency in order-promoting residues, like Asn, Phe, Tyr,
and Trp. This initial examination revealed a preponderance of disordered
regions interpreting ORF4 as proteins consisting of disordered regions, i.e.,
proteins consisting of disordered regions with structured globular domains).
The IDRs (intrinsically disordered regions) as IDPs (intrinsically disordered
proteins)/IDPRs (intrinsically disordered protein regions) play a critical role
in various regulatory functions of viruses, thus were examined that revealed
ORF4 exhibited the characteristics of ORDP (ordered protein), IDPR and IDP.
Further, the identified disorder-based protein binding sites revealed the
involvement of ORF4 in diverse crucial biological functions, substantiating
them as targets of regulation. As ORF4 functions are yet to be completely
explored, thus, our data could help in elucidating its functions. Collectively,
data from this comprehensive investigation suggest the ORF4 protein’s role in
the regulation and pathogenesis of HEV.
Author(s) Details
Zoya Shafat
Centre for Interdisciplinary Research in Basic Sciences,
Jamia Millia Islamia, New Delhi 110025, India.
Please see the link:- https://doi.org/10.9734/bpi/mono/978-81-976932-1-2/CH3
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