Background: Asthma is a chronic lung disease that affects 300 million people worldwide. Allergic asthma is a disease of airway inflammation and hyperresponsiveness. Asthma occurs by activating IgE, a receptor that bears mast cells and basophils, and the triggering factor for this is the inhalation of an allergen when it binds to complementary IgE. And this leads to lung infiltration by eosinophils, neutrophils, then activated (M 2) macrophages and (TH2) lymphocytes, which are inflammatory triggers and all these increase mucus production, which leads to mucosal edema, smooth-muscle hyperresponsiveness, and airway hyperresponsiveness (AHR) due to exposure to e.g. histamine and methacholine.
Objective: The aim of this study was to detect the arginase Ι (ARG
I) enzyme in asthma patients, and clarify its role, in addition to examining
the relationship of this enzyme with bronchial asthma.
Methods: Blood samples were collected from 100 patients from
Department of Medicine in Rizgary Hospital in Erbil City, in addition to intact
100 volunteers; the introduced questionnaire was filled out on the basis of
type-induced asthma, duration of the disease, age of the patients, gender,
family history, and allergy condition, Serum was separated to perform Enzyme-linked immunosorbent assay
(ELISA) in Medical Research Center to examine the association of ARGΙ with
bronchial asthma. By ARG activity we can measure the conversion of arginine to
ornithine and urea. By using a quantitative colorimetric assay at 490 nm,
employing a QuantiChrom arginase assay kit (Bioassay Systems).
Results: Our results depicted the association between ARGΙ and
bronchial asthma: based on their age, significant elevation of serum arginase
level was observed in the patients ≥81 years old, with mean value (100.16±19.77c),
p-value (0.000); also the duration of asthma ≥20 years (82.48±38.81c),
p-value (0.01) were remarkably affected; this significance was found in those
with types of induced asthma and with allergy condition. However
non-significant difference in the frequency of abnormal serum arginase levels
was observed in those patients that have a family history of asthma disease and
gender of the patients. This finding demonstrated a remarkable association of
ARG Ι in the development of asthma at p > 0.05.
Conclusion: The ELISA results indicated that ARG I and bronchial
asthma are remarkably correlated. Patients with asthmatic symptoms have a high
level of ARG Ι (> 40 IU/L).
Author
(s) Details
Ashti Mohammad Amin
Sai
Molecular Biology Department, Medical Research Center / Hawler Medical
University, Kurdistan, Iraq.
hmed Abdulqader
Al-Naqshband
Bacteriology Department, Rizgary Teaching Hospital / Ministry of Health,
Kurdistan, Iraq.
Yasin Kareem Amin
Medical Research Center / Hawler Medical University, Kurdistan, Iraq.
Rebwar Jalal Ali
Biochemistry Department, Medical Research Center / Hawler Medical
University, Kurdistan, Iraq.
Please see the book here:- https://doi.org/10.9734/bpi/dhrd/v6/4419
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