Aims: This study was focused on the assessment and
performance of traditional TB diagnosis methods and new innovative
nanotech-assisted TB methods. The main aim of this study is to undertake the
use of magnetic nanoparticles for TB detection.
Study Design: A total of 400 pulmonary sputum samples of
suspected TB patients were selected for this study out of these 30% (120) were
selected for comparative study. Cross-sectional studies were conducted on
clinical and radiological suspected TB patients.
Place and Duration of Study: A total of one hundred and
twenty clinically suspected TB patients were selected from D Y Patil Medical
College Hospital and Research Institute Kolhapur, for a cross-sectional study
in the month of August 2016 to January 2017. Samples were processed for
diagnosis of TB by ZN stain, culture on L.J. medium and real-time PCR tests.
Methodology: Selection of patient.
Inclusion criteria include clinically diagnosed and
radiologically documented new cases of pulmonary Mycobacterium tuberculosis of
any gender and age group or untreated MTB cases and patients with
anti-tuberculosis drug therapy in the past 6 months. Exclusion criteria include
patients with anti-TB therapy or its constituent drugs in the past 3 months.
The control group was also studied from the patients who had
diagnoses other than pulmonary tuberculosis.
Results: In our present study, 60.83% (73) of patients were
male and 39.17 % (47) were female patients, showing a borderline male
prevalence found in our study. Eighteen percent of patients were found to be in
the age group (21-30 years) and are mostly affected by pulmonary tuberculosis.
MNPs method 99.66% was found to be significant (P < 0.0001**).
Conclusion: In the present study the MNP-DNA extraction
techniques with NALC followed by IS6110 target amplification were found
superior for diagnosis of TB. The MNP-assisted extraction method showed better
results.
Author(s) Details:
Deepak Sawant
Department of Microbiology, D. Y. Patil, Medical College, Hospital
and Research Institute, Kolhapur, 416003, India.
Shivaji Pawar
Centre for Research and Technology Development, Sinhagad Institutes
Solapur, 413255, M. S, India.
Rajendra Patil
Department of Pathology, D. Y. Patil, Medical College, Hospital and Research
Institute, Kolhapur, 416003, India.
Siddhi Sawant
Department of Pathology, D. Y. Patil, Medical College, Hospital and Research
Institute, Kolhapur, 416003, India.
Please see the link here: https://stm.bookpi.org/ACPR-V9/article/view/14368
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