The objective of the study is to separate and identify the
most effective hepatoprotective fraction of methanolic extract of Marsilea
minuta (MMME) by fractionating and evaluating its fractions for
hepatoprotective activity in three mechanistically devised models viz., CCl4,
paracetamol and ethanol-induced liver damage in rats. Excess consumption of
certain drugs like antibiotics, chemotherapeutic agents, acetaminophen, and
exposure to some chemicals such as peroxidised oils, aflatoxin, CCl4, alcohol
etc make the liver vulnerable to a variety of disorders viz., jaundice,
hepatitis etc which are the two major hepatic disorders that account for the
high death rate. An acute toxicity study was carried out on the fractions
according to the Organization for Economic Corporation Development (OECD)-420
guidelines. Liver damage was induced in different groups of rats by
administering 1:1v/v CCl4 in olive oil 1ml/kg.b.w.p.o, 3g/kg.b.w.p.o
paracetamol and 5g/kg.b.w.p.o ethanol and the effect of fractions were tested
for hepatoprotective potential by evaluating serum biochemical parameters,
histology of liver of rats and the most effective bioactive fraction was
screened for its effect on hepatic microsomal drug-metabolizing enzymes (MDMA)
and prothrombin time (PT). It was also tested for its antioxidant properties by
DPPH method, lipid peroxidation method and for detection of different classes
of chemicals present in it. Pretreatment with fractions (toluene, 1-butanol,
aqueous at 50, 100mg/kg.b.w) significantly reversed the changes in serum
biochemical parameters and histology of the liver caused by the three
hepatotoxins namely CCl4, paracetamol and ethanol indicating their
hepatoprotective activity. Research using MMME butanol fraction (BF-MMME)
provided strong evidence for its hepatoprotective properties in PT, DPPH, and
MDMA. While all of the MMME
fractions showed notable hepatoprotective efficacy, the most potent
hepatoprotective fraction was found to be BF-MMME (50 mg/kg). The findings of
the study substantiated the ethnomedicinal value of the plant Marsilea minuta
used in the treatment of hepatitis. However, a comprehensive investigation of
the bioactive fraction BF-MMME is required to identify the active principle(s)
to evaluate the efficacy and toxicity in different models with the mechanism of
action for developing a safe and effective herbal hepatoprotective drug.
Author(s) Details:
Dr. Praneetha Pallerla,
Department of Pharmacognosy and Phytochemistry, University College
of Pharmaceutical Sciences, Kakatiya University, Telangana, India.
Divya Balne
Department of Pharmacognosy and Phytochemistry, University College of
Pharmaceutical Sciences, Kakatiya University, Telangana, India.
Swaroopa Rani Vanapatla
Department of Pharmacognosy and Phytochemistry, University College of
Pharmaceutical Sciences, Kakatiya University, Telangana, India.
Ravi Kumar Bobbala
Department of Pharmacognosy and Phytochemistry, University College of
Pharmaceutical Sciences, Kakatiya University, Telangana, India.
Please see the link here: https://stm.bookpi.org/ACPR-V9/article/view/14364
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