The aim of this study was to evaluate the In vitro
antioxidant activity of the phenolic extracts of olive leaves and olive mill
wastewater and their protective effect against lipid peroxidation. The
antioxidant activity of two varieties (Chemlal and Sigoise) of the tested olive
leaves and olive mill wastewater was evaluated using three different
antioxidant assays (DPPH radical scavenging test, ferric reducing antioxidant
power (FRAP) assay, and hydrogen peroxide scavenging assay). The protective
effect of the tested extracts against lipid peroxidation in an in vitro model
of rat liver homogenate was evaluated through the production of thiobarbituric
acid reactive substances (TBARS). The obtained results showed that the phenolic
extracts of olive leaves and olive mill wastewater are rich in phenolic
compounds mainly flavonoids, with the highest content found in the extract of
Chemlal olive leaves (88.35±0.73 mg GAE/g of extract for polyphenols and
45.98±0.36 mg QE/g of extract for flavonoids). Furthermore, significant
anti-free radical activity and reducing power have been shown by the tested
extracts. In fact, hydrogen peroxide (H2O2) can be neutralized by the studied
extracts. The extract of Chemlal olive leaves exhibited the most important
antioxidant activity. The tested extracts showed a significant decrease in
malonic dialdehyde (MDA) levels and a protective effect against lipid
peroxidation. The high protective effect was exhibited by the phenolic extract
of Chemlal olive leaves. According to the obtained results, it appears that the
phenolic extracts of olive leaves and olive mill wastewater have an important
antioxidant potential and protective effect against lipid peroxidation and they
can be used as natural antioxidant agents in food and pharmaceutical
industries.
Author(s) Details:
Fatiha Abdellah,
Laboratory of Research on Local Animal Products at Ibn Khaldoun
University, Tiaret, Algeria.
Please see the link here: https://stm.bookpi.org/IBS-V6/article/view/14927
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