Chromatographic Finger Printing Analysis in Identification of Phytomarkers by HPTLC Technique | Chapter 9 | Advanced Concepts in Pharmaceutical Research Vol. 2

 Chromatographic fingerprinting methods are becoming more popular and significant in the uniformity of natural fruit. They are employed for two together quantifying marker compounds and authenticating the presence of differing herbs in poly herbal arrangements. Although HPLC and GC are also frequently working for chromatographic fingerprinting of single spice and poly herbal arrangements, HPTLC has been the pioneer in this field. Chromatographic fingerprinting is used to decide the single herbs labeling and qualities before they are contained to herbal formulations. Each herb fated in near future incorporated to the expression must first undergo fingerprinting. This maybe used to identify both the spice and evaluate the main alive components which maybe found in the spice. The final poly herbaceous formulation is developed when all the herbs have happened appropriately treated and their identity and innocence have been confirmed. At this point, a chromatographic means can yield distinctive peaks each of the herbs utilized in herbal expression is used to develop a chromatographic mark of the poly herbal expression. These fingerprints assist in confirming the presence of all the herbs in multi herbaceous preparations. The lack of individual of the distinguishing visage in the chromatographic fingerprint indicates the lack of even a single spice during the production process. In fact, the specific herbs that are present in the formulation can even be distinguished with the aid of a shapely chromatographic fingerprint. This chapter gives an awareness into finger print analysis for labeling of phytomarkers and sophisticated chromatographic means like HPTLC advantages and applications. This phase also gives significance to modern and established method of several origin methods complicated in extraction of phytoconstituents.

Author(s) Details:

Chandrasekar R.,
Department of Pharmacognosy, Faculty of Pharmacy, Pharmaceutical Sciences, Seven Hills College of Pharmacy, Venkatramapuram, Tirupati, Chittoor, Andhrapradesh, India.

Sivagami B.,
Pharmaceutical Sciences, Department of Pharmacognosy, Seven Hills College of Pharmacy, Venkatramapuram, Tirupati, Chittoor, Andhrapradesh, India.

Harshitha S.,
Pharmaceutical Sciences, Department of Pharmacognosy, Seven Hills College of Pharmacy, Venkatramapuram, Tirupati, Chittoor, Andhrapradesh, India.

Anusha G.,
Pharmaceutical Sciences, Department of Pharmacognosy, Seven Hills College of Pharmacy, Venkatramapuram, Tirupati, Chittoor, Andhrapradesh, India.

Rajesh Naik D.,
Pharmaceutical Sciences, Department of Pharmacognosy, Seven Hills College of Pharmacy, Venkatramapuram, Tirupati, Chittoor, Andhrapradesh, India.

Sahul K.,
Pharmaceutical Sciences, Department of Pharmacognosy, Seven Hills College of Pharmacy, Venkatramapuram, Tirupati, Chittoor, Andhrapradesh, India.

Venu B.,
Pharmaceutical Sciences, Department of Pharmacognosy, Seven Hills College of Pharmacy, Venkatramapuram, Tirupati, Chittoor, Andhrapradesh, India.

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