Background: Senna didymobotrya grows naturally in East Africa and is widely used due to the presence of various phytochemicals such as alkaloids, terpenoids, anthraquinones, tannins, saponins, phenols and flavonoids to treat microbial infections in African populations. Antimicrobial properties against bacteria such as Staphylococcus aureus and Bacillus cereus were also found to contain extracts from the candle brush.
Objective: To determine the effectiveness of crude candle brush extract (Senna
didymobotrya) leaves against Aspergillus niger in reducing post-harvest losses
in tomatoes, this study was performed.
Methods: A fully randomized two-treatment design was used, each repeated six
times. In a review of this. The dried leaves were ground into a fine powder and
extracted with 100 percent methanol using the Soxhlet unit. Aspergillus niger
was tested for the minimum inhibitory concentration (MIC) and the minimum
fungicidal concentration (MFC) of the extract. The shelf life of tomatoes was
determined by spraying 0.4 g/ml of the extract reconstituted in water and the
total viable counts were further analyzed at three-day intervals and tested for
mould growth, color changes and odors as indicators of spoilage.
Results: 22 g of crude extract was obtained after extraction from dried leaves
MIC and MFC of crude methanol extract against Aspergillus niger were 0,04 g/ml.
The shelf life of the handled tomatoes was 14 days, compared to 9 days for the
untreated tomatoes. T-test findings at (P ⁇ 0.05)
Based on the total viable counts, a substantial difference was seen between the
treated and untreated samples.
Author (s) Details
Dr. L. G. Njue
Department of Food Science, Nutrition and Technology, University of Nairobi, Kenya.
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