Antimicrobial Activity of Poly(ester urea) Electrospun Fibers Loaded with Bacteriophages | Chapter 8 | Current Perspectives on Chemical Sciences Vol. 5

 The capacity of two representative biocompatible polymers to load bacteriophages into electrospun nanofibers was evaluated, paying particular attention to the possibility of retaining their antibacterial function. Specifically, the work involves the following steps: (a) evaluation of the impact of the electric field applied on the phage operation; (b) evaluation of the activity where the process of lyophilization may be prevented by the use of water-soluble polymers (e.g. poly(ethylene glycol); (c) evaluation of the activity when organic solvent is needed for the dissolution of the polymer and theoretical lyophilization is theoretical A poly(ester urea) (PEU) derived from the natural L-leucine amino acid was considered in this case. It has been shown that the adsorption of commercial bacteriophage preparations into calcium carbonate particles is a promising technique to prevent lyophilization and keep the initial operation of the bactericide to a limit. Due to their particular activity against Staphylococci (e.g., S. aureus) and Streptococci (e.g., S. pyogenes) bacteria, the Phagestaph and Fersis bacteriophage commercial preparations were selected for this analysis. The biocompatibility of both unloaded and bacteriophage-loaded PEU scaffolds was demonstrated by adhesion and proliferation assays using epithelial cells, although some minor variations were observed depending on the form of bacteriophage and the selected preparation methodology.

Author(s) Details

Angélica Díaz
Departament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.

Luis J. del Valle
Departament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.

Noel Rodrigo
Departament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.

María Teresa Casas
Departament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.

George Chumburidze
Center for Medical Biotechnology & Bioengineering, Georgian Technical University, 77 Kostava str., Tbilisi 0175, Georgia.

Ramaz Katsarava
Center for Medical Biotechnology & Bioengineering, Georgian Technical University, 77 Kostava str., Tbilisi 0175, Georgia and Institute of Chemistry and Molecular Engineering, Agricultural University of Georgia, # 240 David Aghmashenebeli Alley, Tblisi 0159, Georgia.

Jordi Puiggalí

Departament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain and Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.

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