Development and Characterisation of PLA-Nanofiber Scaffolds with Cinnamon Oil-Lanolin Emulsions for Antibacterial Human Skin Grafts | Chapter 1 | Chemical and Materials Sciences: Research Findings Vol. 4

 

Tissue engineering has emerged as an innovative solution to develop alternatives to traditional grafts, particularly with scaffolds. These structures, intended for use as skin grafts, must meet specific requirements, including biocompatibility, a porous structure, mechanical properties similar to human skin, and antibacterial capabilities. The interest in tissue engineering is growing in the scientific and medical communities, as it can solve actual problems regarding donor tissues, wound healing and drug delivery systems. Nanofibers are gaining relevance in this topic thanks to their excellent mechanical properties and similarities to the human skin.

 

Polylactic acid (PLA) as polymer and Hexafluoro isopropanol (HFIP) as solvent, both from Sigma-Aldrich, were used in the membrane formation. This project has explored how combining the nanofibers’ membranes created of PLA, made by electrospinning, with a dissolution of lanoline and cinnamon essential oil not only imitates the human skin, as it was demonstrated in a later project, but also obtains an antibacterial character. Analytical techniques such as a spectrophotometer, an electrokinetic analyser, a scanning electron microscope, a Fourier transform infrared spectroscope, and an optical tensiometer were employed.

 

Results confirmed successful integration and migration of the cinnamon oil, with antibacterial efficacy achieved against specific bacterial strains, as hypothesised. Notably, scaffolds composed of seven layers exhibited migration behaviour closely aligned with theoretical expectations. Additionally, it has also been found that the scaffold presents an antibacterial character when analysing the Escherichia coli bacteria.

 

In conclusion, among all the analytical methods used, the drug delivery test and the FT-IR test have been the most useful in order to determine the migration of the emulsion. With the drug delivery test, it could also be determined how the migration of LAT changes depending on the number of layers used to form the scaffolds.

 

 

Author (s) Details

Leyre Marqués Arribas
Universitat Politècnica de Catalunya, Terrassa, Spain.

 

Manuel José Lis Arias
Institute of Textile Research and Industrial Cooperation of Terrassa-INTEXTER, Universitat Politècnica de Catalunya, Colom 15, 08222 Terrassa, Spain.

 

Francisco Cano
Institute of Textile Research and Industrial Cooperation of Terrassa-INTEXTER, Universitat Politècnica de Catalunya, Colom 15, 08222 Terrassa, Spain and Department of Materials Science and Engineering, Universitat Politècnica de Catalunya, Colom 1, 08222 Terrassa, Spain.

 

Meritxell Martí Gelabert
Institute of Textile Research and Industrial Cooperation of Terrassa-INTEXTER, Universitat Politècnica de Catalunya, Colom 15, 08222 Terrassa, Spain and Department of Materials Science and Engineering, Universitat Politècnica de Catalunya, Colom 1, 08222 Terrassa, Spain.

 

Alba Martínez
nstitute of Textile Research and Industrial Cooperation of Terrassa-INTEXTER, Universitat Politècnica de Catalunya, Colom 15, 08222 Terrassa, Spain.

 

Manuel José Lis Arias
Institute of Textile Research and Industrial Cooperation of Terrassa-INTEXTER, Universitat Politècnica de Catalunya, Colom 15, 08222 Terrassa, Spain.

 

 

Please see the book here:- https://doi.org/10.9734/bpi/cmsrf/v4/5238

Antimicrobial Activity of Poly(ester urea) Electrospun Fibers Loaded with Bacteriophages | Chapter 8 | Current Perspectives on Chemical Sciences Vol. 5

 The capacity of two representative biocompatible polymers to load bacteriophages into electrospun nanofibers was evaluated, paying particular attention to the possibility of retaining their antibacterial function. Specifically, the work involves the following steps: (a) evaluation of the impact of the electric field applied on the phage operation; (b) evaluation of the activity where the process of lyophilization may be prevented by the use of water-soluble polymers (e.g. poly(ethylene glycol); (c) evaluation of the activity when organic solvent is needed for the dissolution of the polymer and theoretical lyophilization is theoretical A poly(ester urea) (PEU) derived from the natural L-leucine amino acid was considered in this case. It has been shown that the adsorption of commercial bacteriophage preparations into calcium carbonate particles is a promising technique to prevent lyophilization and keep the initial operation of the bactericide to a limit. Due to their particular activity against Staphylococci (e.g., S. aureus) and Streptococci (e.g., S. pyogenes) bacteria, the Phagestaph and Fersis bacteriophage commercial preparations were selected for this analysis. The biocompatibility of both unloaded and bacteriophage-loaded PEU scaffolds was demonstrated by adhesion and proliferation assays using epithelial cells, although some minor variations were observed depending on the form of bacteriophage and the selected preparation methodology.

Author(s) Details

Angélica Díaz
Departament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.

Luis J. del Valle
Departament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.

Noel Rodrigo
Departament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.

María Teresa Casas
Departament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.

George Chumburidze
Center for Medical Biotechnology & Bioengineering, Georgian Technical University, 77 Kostava str., Tbilisi 0175, Georgia.

Ramaz Katsarava
Center for Medical Biotechnology & Bioengineering, Georgian Technical University, 77 Kostava str., Tbilisi 0175, Georgia and Institute of Chemistry and Molecular Engineering, Agricultural University of Georgia, # 240 David Aghmashenebeli Alley, Tblisi 0159, Georgia.

Jordi Puiggalí

Departament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain and Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.

View Book :- https://bp.bookpi.org/index.php/bpi/catalog/book/354