The capacity of two representative
biocompatible polymers to load bacteriophages into electrospun nanofibers was
evaluated, paying particular attention to the possibility of retaining their
antibacterial function. Specifically, the work involves the following steps:
(a) evaluation of the impact of the electric field applied on the phage
operation; (b) evaluation of the activity where the process of lyophilization
may be prevented by the use of water-soluble polymers (e.g. poly(ethylene
glycol); (c) evaluation of the activity when organic solvent is needed for the
dissolution of the polymer and theoretical lyophilization is theoretical A
poly(ester urea) (PEU) derived from the natural L-leucine amino acid was
considered in this case. It has been shown that the adsorption of commercial
bacteriophage preparations into calcium carbonate particles is a promising
technique to prevent lyophilization and keep the initial operation of the
bactericide to a limit. Due to their particular activity against Staphylococci
(e.g., S. aureus) and Streptococci (e.g., S. pyogenes) bacteria, the Phagestaph
and Fersis bacteriophage commercial preparations were selected for this
analysis. The biocompatibility of both unloaded and bacteriophage-loaded PEU
scaffolds was demonstrated by adhesion and proliferation assays using
epithelial cells, although some minor variations were observed depending on the
form of bacteriophage and the selected preparation methodology.
Author(s) Details
Angélica Díaz
Departament
d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2,
C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.
Luis J. del Valle
Departament
d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2,
C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.
Noel Rodrigo
Departament
d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2,
C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.
María Teresa Casas
Departament
d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2,
C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.
George Chumburidze
Center
for Medical Biotechnology & Bioengineering, Georgian Technical University,
77 Kostava str., Tbilisi 0175, Georgia.
Ramaz Katsarava
Center for Medical Biotechnology &
Bioengineering, Georgian Technical University, 77 Kostava str., Tbilisi 0175,
Georgia and Institute of Chemistry and Molecular Engineering, Agricultural
University of Georgia, # 240 David Aghmashenebeli Alley, Tblisi 0159, Georgia.
Jordi Puiggalí
Departament d’Enginyeria Química, EEBE, Universitat Politècnica de
Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain and
Research Center for Multiscale Science and Engineering, Universitat Politècnica
de Catalunya, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain.
View Book :- https://bp.bookpi.org/index.php/bpi/catalog/book/354
