Histopathological Assessment of Intestinal Morphology in Japanese Quails Following Essential Oil Supplementation | Chapter 9 | Research Perspective on Biological Science Vol. 10

 

Among livestock sub-sectors, the poultry industry is the most organised and scientifically advanced, and has emerged as the fastest-growing segment of Indian agriculture. Essential oils, derived from herbs and medicinal plants, have gained considerable attention as potential alternatives to antibiotic growth promoters due to their antimicrobial, antioxidant, immunomodulatory, and digestive stimulant properties. Despite the documented benefits of essential oils in broilers, limited information is available on their effects on growth performance and nutrient utilisation in Japanese quails under Indian conditions. The present study evaluated the effect of dietary supplementation of cinnamon and peppermint essential oils on intestinal histomorphology in Japanese quails. Two hundred day-old chicks were randomly assigned to five dietary treatments: basal diet (control), cinnamon oil (500 ppm), peppermint oil (500 ppm), a combination of both oils (250 ppm each), and a higher-level combination (500 ppm each) for 35 days. Jejunal samples were collected at the end of the trial for histological examination. Villus height, crypt depth, and villus height to crypt depth ratio were significantly (p<0.05) influenced by essential oil supplementation. Treated groups showed increased villus height and improved villus–crypt ratio compared to control, indicating enhanced intestinal structural development. The combined supplementation, particularly at higher inclusion levels, produced superior morphometric responses. These findings suggest that cinnamon and peppermint essential oils beneficially modulate intestinal architecture in Japanese quails.

 

 

Author(s) Details

Abhishek Tiwari
Animal Nutrition Division, ICAR–National Dairy Research Institute (NDRI), Karnal–132001, Haryana, India.

 

Debashis Roy
Department of Animal Nutrition, College of Veterinary & Animal Sciences, SVPUAT, Meerut - 250110, Uttar Pradesh, India.

 

Ajit Kumar
Department of Animal Nutrition, College of Veterinary & Animal Sciences, SVPUAT, Meerut - 250110, Uttar Pradesh, India.

 

Ram Kumar Singh
Department of Livestock Farm Complex (Animal Nutrition), College of Veterinary & Animal Sciences, SVPUAT, Meerut - 250110, Uttar Pradesh, India.

 

Vikas Jaiswal
Department of Veterinary Pathology, College of Veterinary & Animal Sciences, SVPUAT, Meerut-250110, Uttar Pradesh, India.

 

Manoj Kumar Singh
Department of Livestock Production Management, College of Veterinary & Animal Sciences, SVPUAT, Meerut-250110, Uttar Pradesh, India.

 

Harshit Verma
Department of Veterinary Microbiology, College of Veterinary & Animal Sciences, SVPUAT, Meerut-250110, Uttar Pradesh, India.

 

Shubham Nayak
Department of Animal Reproduction, Gynaecology and Obstetrics, ICAR-National Dairy Research Institute (NDRI), Karnal-132001, Haryana, India.

 

Kaushlendra Narayan Dwivedi
Department of Animal Nutrition, College of Veterinary & Animal Sciences, SVPUAT, Meerut - 250110, Uttar Pradesh, India.

 

Priyanka Rani
Department of Livestock Production Management, College of Veterinary & Animal Sciences, SVPUAT, Meerut-250110, Uttar Pradesh, India.

 

Rahul Tiwari
Division of Animal Nutrition, ICAR-National Dairy Research Institute (NDRI), Karnal-132001, Haryana, India.

 

Vaidehi Mishra
Department of Livestock Production Management, College of Veterinary & Animal Sciences, SVPUAT, Meerut-250110, Uttar Pradesh, India.

 

Gagan Chawla
Animal Physiology Division, ICAR-National Dairy Research Institute (NDRI), Karnal-132001, Haryana, India.

 

Please see the book here :- https://doi.org/10.9734/bpi/rpbs/v10/7254

Development and Characterisation of PLA-Nanofiber Scaffolds with Cinnamon Oil-Lanolin Emulsions for Antibacterial Human Skin Grafts | Chapter 1 | Chemical and Materials Sciences: Research Findings Vol. 4

 

Tissue engineering has emerged as an innovative solution to develop alternatives to traditional grafts, particularly with scaffolds. These structures, intended for use as skin grafts, must meet specific requirements, including biocompatibility, a porous structure, mechanical properties similar to human skin, and antibacterial capabilities. The interest in tissue engineering is growing in the scientific and medical communities, as it can solve actual problems regarding donor tissues, wound healing and drug delivery systems. Nanofibers are gaining relevance in this topic thanks to their excellent mechanical properties and similarities to the human skin.

 

Polylactic acid (PLA) as polymer and Hexafluoro isopropanol (HFIP) as solvent, both from Sigma-Aldrich, were used in the membrane formation. This project has explored how combining the nanofibers’ membranes created of PLA, made by electrospinning, with a dissolution of lanoline and cinnamon essential oil not only imitates the human skin, as it was demonstrated in a later project, but also obtains an antibacterial character. Analytical techniques such as a spectrophotometer, an electrokinetic analyser, a scanning electron microscope, a Fourier transform infrared spectroscope, and an optical tensiometer were employed.

 

Results confirmed successful integration and migration of the cinnamon oil, with antibacterial efficacy achieved against specific bacterial strains, as hypothesised. Notably, scaffolds composed of seven layers exhibited migration behaviour closely aligned with theoretical expectations. Additionally, it has also been found that the scaffold presents an antibacterial character when analysing the Escherichia coli bacteria.

 

In conclusion, among all the analytical methods used, the drug delivery test and the FT-IR test have been the most useful in order to determine the migration of the emulsion. With the drug delivery test, it could also be determined how the migration of LAT changes depending on the number of layers used to form the scaffolds.

 

 

Author (s) Details

Leyre Marqués Arribas
Universitat Politècnica de Catalunya, Terrassa, Spain.

 

Manuel José Lis Arias
Institute of Textile Research and Industrial Cooperation of Terrassa-INTEXTER, Universitat Politècnica de Catalunya, Colom 15, 08222 Terrassa, Spain.

 

Francisco Cano
Institute of Textile Research and Industrial Cooperation of Terrassa-INTEXTER, Universitat Politècnica de Catalunya, Colom 15, 08222 Terrassa, Spain and Department of Materials Science and Engineering, Universitat Politècnica de Catalunya, Colom 1, 08222 Terrassa, Spain.

 

Meritxell Martí Gelabert
Institute of Textile Research and Industrial Cooperation of Terrassa-INTEXTER, Universitat Politècnica de Catalunya, Colom 15, 08222 Terrassa, Spain and Department of Materials Science and Engineering, Universitat Politècnica de Catalunya, Colom 1, 08222 Terrassa, Spain.

 

Alba Martínez
nstitute of Textile Research and Industrial Cooperation of Terrassa-INTEXTER, Universitat Politècnica de Catalunya, Colom 15, 08222 Terrassa, Spain.

 

Manuel José Lis Arias
Institute of Textile Research and Industrial Cooperation of Terrassa-INTEXTER, Universitat Politècnica de Catalunya, Colom 15, 08222 Terrassa, Spain.

 

 

Please see the book here:- https://doi.org/10.9734/bpi/cmsrf/v4/5238