Wednesday, 14 October 2020

Investigation on in vitro Evaluation of Antioxidant and Anti-inflammatory Properties of Methanol and Dichloromethane Extracts of the Leaf of Globimetula oreophila | Chapter 11 | Current Research and Development in Chemistry Vol. 4

 

Objectives: The purpose of the study was to investigate the antioxidant and membrane stabilising potential of Globimetula oreophila leaves in order to provide scientific support for the plant 's existing ethnomedicinal or traditional uses. Methodology: The research included the collection, identification, drying, spraying, extraction and fractionation of dried G leaf extracts. Oreophila. Oreophil. In the assays (2,2-diphenyl-1-picrylhydrazyl hydrate, nitric oxide, total antioxidant potential and ferric reducing antioxidant power), the anti-inflammatory and antioxidant activities of the extracts and fractions were evaluated using the red blood cell membrane stabilisation technique.

Results: The findings showed that the extracts and the fractions of G. Oreophila demonstrated high antioxidant activity. The highest antioxidant activity with IC50 of 45.83 ± 0.13 μg / ml was shown in fraction 1 of the methanol extract, while fraction 7 of the same extract was the most potent in stabilising stressed red blood cells with 100 ± 0.00 percent stability of the membrane. The operations of both the fractions and the G. extracts. Compared to regular antioxidants and anti-inflammatory drugs, oreophila is optimistic. In addition, the operations of the fractions and extracts are dependent on concentration. Conclusion: The analysis showed that G's extracts and fractions. Oreophila has and exhibits anti-inflammatory and antioxidant functions that are concentration-dependent and beneficial compared with those of anti-inflammatory nonsteroidal drugs. It could be concluded on the basis of these findings that the extracts and the fractions of G. Oreophila includes oreophila, which Principles capable of stabilising the exposure of bovine red blood cell membranes to heat and hypotonic lyses could also serve as clinical evidence for the use of these plant extracts by conventional local health professionals in the management and treatment of inflammatory diseases.

Author (s) Details

Dr. Esther O. Faboro
Department of Chemistry, Bowen University, Iwo, Nigeria.

Mr. Idowu J. Olawuni
Department of Biochemistry, Obafemi Awolowo University, Ile Ife, Nigeria.

Dr. Bolajoko A. Akinpelu
Department of Biochemistry, Obafemi Awolowo University, Ile Ife, Nigeria.

Professor Oluokun O. Oyedapo
Department of Biochemistry, Obafemi Awolowo University, Ile Ife, Nigeria

Professor Ezekiel O. Iwalewa
Department of Pharmacology and Therapeutics, University of Ibadan, Ibadan, Nigeria.

Professor Craig A. Obafemi
Department of Chemistry, Obafemi Awolowo University, Ile Ife, Nigeria.

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https://bp.bookpi.org/index.php/bpi/catalog/book/282

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