Using disc diffusion (DD) and agar well diffusion (AWD) methods, the combination of treatment of crude turmeric rhizome extracts with potash alum against four ( 4) bacterial and five (5) fungal species was investigated. Extracts with or without potash alum (PTA / Alum) were dose-dependently sensitive against all test microbes by inhibiting their growth. The largest inhibition zone (DIZ) diameter was obtained with ethanolic turmeric extract (ETE) and potash alum (ETE+PTA) at a concentration of 0.3 g on Gram-negative bacteria such as Escherichia coli (16.6±0.8 mm) and Pseudomonas fluorescens (15.3±1.1 mm) and Gram-positive bacteria, Staphylococcus aureus (15.0±0.0 mm), whereas potash alum with the greatest inhibition activity was obtained with strong antifungal activity. (17±1.0 mm) flavus, S. (14 mm) and C. cerevisiae. Albicans (12±1.0 mm , respectively) by DD. The best inhibition was shown by AWD bioassay, with PTA Against Bacillus cereus activity (17.8±1.0 mm), S. Aureus (16.0 ± 0.7 mm) and, on P. fluorescens and E., 14.0 mm. Whereas coli, ETE+PTA showed the highest antifungal activity on A. Terreus (35±1.0 mm), Crystallium of Penicillium and A. 33.0 mm (flavus) and S. Cerevisiae, respectively (24.0 mm). Additionally, the apparent demonstration of antibacterial activity by extracts of turmeric rhizome and potash alum on both Gram positive and Gram negative bacteria as well as against fungi is indicative of wide spectrum activity. However, on the other hand, the high activity of Ofloxacin (OFL) and Ketoconazole (KTA) against test microbes underlines their dominance with or without PTA / alum extracts. However, the increase in bioactivity of turmeric rhizome extracts was accentuated by the incorporation of potash alum, and such novel combinational approaches to research with healthy, natural materials will provide animals and human pathologies with reliable alternatives to synthetic antimicrobial chemotherapy.
Author (s) Details
Dr. Lawrence O. Amadi
Department of Microbiology, Faculty of Science, Rivers State University, P.M.B. 5080, Nkpolu-Oroworukwo, Port Harcourt, Nigeria and Department of Microbiology, School of Applied Science, Ken Saro-Wiwa Polytechnic, P.M.B. 20, Bori, Rivers State, Nigeria.
Joy S. Ekechi
Department of Microbiology, Faculty of Science, Rivers State University, P.M.B. 5080, Nkpolu-Oroworukwo, Port Harcourt, Nigeria.
Seth M. Akporutu
Department of Microbiology, Faculty of Science, Rivers State University, P.M.B. 5080, Nkpolu-Oroworukwo, Port Harcourt, Nigeria.
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