Determination of Chlorogenic Acid and Sesquiterpene Lactone Content in Root, Flour and Roasted Grains of Industrial Chicory | Chapter 4 | Recent Advances in Science and Technology Research Vol. 6

 

The secondary metabolites of industrial chicory sesquiterpene lactones (STL) and chlorogenic acids (CGA) indicate potential nutritional and organoleptic added value for this crop. They are soluble major secondary metabolites that accumulate in chicory, particularly in Asteraceae. Their quantification was defined here in chicory root and its dried (flour) and roasted (grain) types, as a quality parameter. For all chicory root products, the technique uses one extraction and one analysis. Different low to high polarity solvents, such as methanol, chloroform, or n-hexane, were examined alone, in different amounts in combination, or with an acidified or neutral aqueous solvent. The best extraction yield, 21 percent greater than alcohol mixtures, was produced by the water / chloroform / methanol (30/30/40, v / v / v) mixture. Strong retention time and repeatability of the region (RSD mean percentage 0.46 and 5.6, respectively) and linearity (almost 2 per 0.96) were achieved. The calculated levels of STL and chlorogenic acid were 254.7 and 100.2 g / g in root dry matter, 792.5 and 1.547 g / g in flour, and 160.4 and 822.5 g / g in roasted grains, respectively. This method is a quick, reproducible and straightforward way of quantifying chlorogenic acids and STL in chicory raw material and end products with an average recovery of 106 percent and accuracy of 90 percent.

Author (s) Details

Honorine Willeman
BioEcoAgro, Joint Research Unit 1158, University Lille, University Liège, UPJV, YNCREA, University Artois, University Littoral Côte d’Opale, INRAE, ICV – Institut Charles Viollette, F-59000 Lille, France.

Philippe Hance
BioEcoAgro, Joint Research Unit 1158, University Lille, University Liège, UPJV, YNCREA, University Artois, University Littoral Côte d’Opale, INRAE, ICV – Institut Charles Viollette, F-59000 Lille, France and Joint Unit University Lille -Florimond-Desprez "CHIC41Health", F-59000 Lille, France.

Anne Fertin
Polytech'Lille GB-IAAL, F-59000 Lille, France.

Najia Voedts
BioEcoAgro, Joint Research Unit 1158, University Lille, University Liège, UPJV, YNCREA, University Artois, University Littoral Côte d’Opale, INRAE, ICV – Institut Charles Viollette, F-59000 Lille, France.

Nathalie Duhal
University Lille, CHU Lille, ULR 7365-GRITA-Groupe de Recherche sur les formes Injectables et les Technologies Associées, Plateau de spectrométrie de Masse, F-59000 Lille, France.

Jean-François Goossens
University Lille, CHU Lille, ULR 7365-GRITA-Groupe de Recherche sur les formes Injectables et les Technologies Associées, Plateau de spectrométrie de Masse, F-59000 Lille, France.

Professor Jean-Louis Hilbert
BioEcoAgro, Joint Research Unit 1158, University Lille, University Liège, UPJV, YNCREA, University Artois, University Littoral Côte d’Opale, INRAE, ICV – Institut Charles Viollette, F-59000 Lille, France and Joint Unit University Lille -Florimond-Desprez "CHIC41Health", F-59000 Lille, France.

View Book :- https://bp.bookpi.org/index.php/bpi/catalog/book/281