Background and Aim: Viscum album (mistletoe) is a hemi-parasitic
shrub, frequently globular in shape. Viscum album is well recognized as a
medicinal plant due to its beneficial effects, including anti-tumor activity,
antioxidant, anti-inflammatory, anti-hepatotoxic, hypoglycemic, and
antimicrobial properties as well as for lowering blood pressure. This study
aims to investigate the effect of time of collection on the antioxidant values
of mistletoes (Viscum album) grown on orange trees.
Methods: Aqueous leaf extracts of different times of harvest of
Viscum album were prepared and standard methods were used to determine
quantitatively the antioxidants values in the plant extracts. The fresh leaves
of the mistletoe plant (Viscum album) from the host plant Orange tree were
obtained from a research farm of Adeyemi College of Education, Ondo, Ondo
State, Nigeria. The total phenolic content was determined using the
Folin-Ciocalteau reagent. The free radical scavenging activities of aqueous
extract were measured with stable 1, 1-diphenyl- 2-picryl hydrazyl radical
(DPPH) spectrophotometrically.
Results: In the experiments, the total phenol content in aqueous
leaves extracts of Viscum album was 19.5840 mg/g, at 6: 00 am, 55.4061 mg/g at
1:00 pm and the value 86.3682 mg/g recorded at 7:00 pm. The value of 0.344 mg/g
was recorded for flavonoids at 6:00 am while the value of 0.676 mg/g was
observed at 1:00 pm and at 7:00 pm it was 1.458 mg/g. The % of inhibition of
DPPH content in aqueous leaves extracts of Viscum album was 36.819 mg/g, at 6:
00 am, 49.213 mg/g at 1:00 pm and the value of 61.203 mg/g was recorded at 7:00
pm. The antioxidants values were significant (P =0.05) when compared time of
plant collection.
Conclusion: The results showed that the antioxidant values depend
on the time of collection which was maximum when the leaves were obtained at
dawn. It is therefore advisable that the use of this extract in herbal medicine
should be encouraged and the plant should be plucked at dawn to maximise the
antioxidant potential of the mistletoes.
Author(s)
Details
Akinlami
O. Omokehinde
Department of Chemistry, Adeyemi Federal University of Education,
Ondo, Nigeria.
Hammed
O. Oloyede
Department of Chemistry, Adeyemi Federal University of Education,
Ondo, Nigeria.
Please see the book here:- https://doi.org/10.9734/bpi/prrat/v4/276
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