Studies on Genetic Diversity and Polymorphism of Acacia constricta and Acacia farnesiana | Chapter 6 | Research Advances in Microbiology and Biotechnology Vol. 8

 In this study, the ancestral diversity and variety of the A. farnesiana and Acacia constricta species were examined utilizing randomly intensified polymorphic DNA (RAPD). Several groups have stated on the utility of RAPD tombstones as a source of phylogenetic facts.  The seeds of 10 types of A. constricta and A. farnesiana were randomly picked from five districts (Namakkal, Perambalur, Nagapattinam, Ramanathapuram and Pudukkottai) of Tamil Nadu, India. Analyses using RAPD dossier revealed 87.5% variety.  The genetic nearness between assortments of both species categorized from 29 to 93%. Between the species, the GT-8 difference of A. constricta and GCT-2 of A. farnesiana had the lowest historical similarity (29%), but the GT-8 and GT-6 varieties of A. constricta had ultimate genetic correspondence (41%), with the difference GCT-3 of A. farnesiana.  Polymorphism was found in 51% of the fragments exaggerated with 22 SSR primers. The genetic likeness between cultivars of two together species categorized from 20 to 91%.  The first cluster was exhibited by three A. constricta types, when in fact the second cluster was revealed by seven A. farnesiana species accompanying two sub clusters, and GCT-3 was connected separately to the main cluster.  The test of genetic similarities and assembling patterns between RAPD and SSRs told almost 85% correspondence.  SSR analysis has, the differences Cocker-1, GCT-2 and GC-1 are grouped together with close hereditary similarity when distinguished to the grouping pattern of differences in RAPD analysis. In variants refined in Tami Nadu, isoenzyme analysis of the peroxidase and polyphenol oxidase enzymes told species-particular bands as well as bands distinguishing to the Acacia genus. In analyzing the difference of Acacia species, these main data grant permission aid researchers in identifying duplicate accessions.

Author(s) Details:

M. Balasubramanian,
Department of Biotechnology, Vivekanandha Arts and Science College for Women, Veerachipalayam, Sankagiri West, Salem District, Tamil Nadu State, India.

S. Janaki,
Department of Microbiology, Vivekanandha College of Arts and Sciences for Women (Autonomous), Elayampalayam, Tiruchengode, Namakkal District, Tamil Nadu State, India.

M. Abinaya,
Department of Biotechnology, Vivekanandha Arts and Science College for Women, Veerachipalayam, Sankagiri West, Salem District, Tamil Nadu State, India.

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