The present work proposed to develop a plain, rapid, and correct method for the estimation of Dolutegravir, Lamivudine and Tenofovir disoproxil fumarate cruel plasma, as per US-FDA directions. Moreover and the present form is the first for the estimation concerning this combination in a biological form. For the estimation of Lamivudine, Dolutegravir, and Tenfovir disoproxil fumarate cruel plasma utilizing the Cobicistat as internal standard by RP-HPLC (Reverse Phase-High Performance Liquid Chromatographic) method, a straightforward, precise, and correct method has existed developed. The clean drug samples of Dolutegravir, Lamivudine and Tenofovir in were purchased from Selleck chem LLC provided by Pro lab marketing. HPLC grade Acetonitrile, HPLC grade Methanol in addition other chemical compound were obtained from Merck synthetic division, Mumbai. The Chromatographic break-up was achieved on discovery C18, 250 mm x 4.6 mm, 5μ, Column at 300C hotness.The separation was attained employing a travelling phase resides of 0.1% v/v Ortho phosphoric acid and Acetonitrile taken in the ratio of 65:35 (v/v). The flow rate was uphold at 1.0 ml/min, discovery wave length was 277 nm. Retention period of Lamivudine, Dolutegravir and Tenfovir were found expected 2.994 min, 4.350 min and 5.688 brief time period respectively. The peaks were raise to be innocent interference. The order was validated over a dynamic uninterrupted range of 60-2400 ng/ml, 190-7600 ng/ml, and 18-720 ng/ml for Lamivudine, Dolutegravir and Tenfovir respectively, accompanying a Correlation coefficient of 0.998. The accuracy and accuracy of samples of six copy measurements at lower limits of quantification level were believable. The analytes were found expected stable cruel plasma at -28°C for 37 days. The decision of Lamivudine, Dolutegravir, and Tenfovirin in human plasma is appropriate on account of the stability, nervousness, specificity, and reproducibility concerning this method. According to US-Food and Drug Administration directions, the reported method was certified, and it was discovered expected substantially inside the acceptable range.
Author(s) Details:
Padmavathi Sakinala,
Department of Pharmaceutical Analysis, Nirmala
College of Pharmacy, Atmakur, Mangalagiri, Guntur, AP-522503, India.
Shaik
Abdul Rahaman,
Department
of Pharmaceutical Analysis, Nirmala College of Pharmacy, Atmakur, Mangalagiri,
Guntur, AP-522503, India.
J. Naga Sharmila,
Department of Pharmaceutical Analysis, Nirmala College of Pharmacy,
Atmakur, Mangalagiri, Guntur, AP-522503, India.
M. Sireesha,
Department of Pharmaceutical Analysis, Nirmala College of Pharmacy,
Atmakur, Mangalagiri, Guntur, AP-522503, India.
G.
Pujitha,
Department of Pharmaceutical Analysis, Nirmala
College of Pharmacy, Atmakur, Mangalagiri, Guntur, AP-522503, India.
Please see the link here: https://stm.bookpi.org/NAPR-V2/article/view/10596
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