First and Second Order Derivative Spectrophotometric Methods for Simultaneous Determination of Emtricitabine and Tenofovir Disoproxil Fumarate Tablets | Chapter 5 | Progress in Chemical Science Research Vol. 9

 Emtricitabine and tenofovir disoproxil fumarate in clean and tablet quantity forms are together estimated utilizing first and second order derivative spectrophotometric designs devised in the current study. Emtricitabine and tenofovir disoproxil Fumarate were linked to create a standard/sample, and the ranges of the incorporation, first order, and second order derivatives were written distinguished to a reagent blank. With absorbance principles of 0.639, 0.420, and 0.164, respectively, three peaks at 265 and 215 nm and individual lowland at 235 nm were noted in the incorporation spectra. In the first order derivative range, three definite peaks at 380, 325, and 260 nm and three negative peaks at 355, 280, and 225 nm were identified. The peak at 260 nm and the basin at 280 nm have the chief positive and negative amplitudes, individually, compared to the different highs and lows.  In the second order derivative spectrum, three peaks at 365, 290 and 250 nm accompanying best positive size at 250 nm and three valleys at 350, 265 and 215 nm accompanying maximum negative size at 215 nm were noted. In order to substantiate the approach for tenofovir disoproxil fumarate and emtricitabine, certain amplitudes at 282.4 nm and negative amplitudes at 258.7 nm in the combined first derivative range, respectively, were calculated.  Emtricitabine was ratified using the size of the second derivative sharp peak at 282.4 nm in the case of the second derivative pattern. Emtricitabine interferes with calculations of tenofovir disoproxil Fumarate at 258.7 nm, so the difference 'tween two together amplitudes was used to validate the procedure for tenofovir disoproxil Fumarate. System and procedure accuracy RSD percentages were raise to be inside satisfactory bounds. Accuracy trials at three spiking levels designated that the mean recovery allotment categorized from 99.4 to 100.48.  The proposed plans were used to decide the Truvada assay, and it was discovered that the allotment of assay for emtricitabine and tenofovir disoproxil Fumarate, respectively, was in the range of 100.75 ± 0.534 and 99.46 ± 0.671.   The grown plans were found expected exact, accurate, undeviating and sensitive.

Author(s) Details:

B. Valli Purnima,
Department of Chemistry, Acharya Nagarjuna University, Guntur, India and Department of Chemistry, Sir C. R. Reddy College for Women, Vatluru, Eluru, India.

G. Ramu,
Department of Chemistry, Sir C. R. Reddy College-Autonomous, Eluru, India.

D. Ramachandran,
Department of Chemistry, Acharya Nagarjuna University, Guntur, India.

Please see the link here: https://stm.bookpi.org/PCSR-V9/article/view/10699