In African traditional medicine, the plants Lantana ukambensis (Vatke) Verdc. (Verbenaceae) and Balanites aegyptiaca (L.) Delile (Zygophyllaceae) are used to treat wounds and inflammatory diseases. The aim of this study is to see how the extracts and fractions of these plants affect HCT-116 and HT-29 cells in terms of cytotoxicity, cell cycle arrest, and pro-apoptotic impact. In this analysis, MTS, crystal violet, Hoechst staining, annexin V/PI, and cytometry were used. HCT-116 and HT-29 cells were substantially cytotoxic when crude extracts of these plants were used. On HCT-116, the 1-butanol and ethyl acetate fractions of L. ukambensis reduced the G1 phase by 20.53 percent and 28.47 percent, respectively, and raised the G2/M phase by 23.47 percent and 25.90 percent. Furthermore, after 48 hours of exposure, the 1-butanol fraction increased the cumulative value of apoptotic cells by 49.77 percent on HCT-116, while the ethyl acetate fraction increased it by 53.37 percent at 15 g/mL. The ethyl acetate fraction increased G1 phase by 3.83 percent on HCT-116 and 8.6 percent on HT-29 in B. aegyptiaca, thus decreasing G2/M phase by 5.63 percent on HCT-116 and 6.62 percent on HT-29. Furthermore, HCT-116 increased the number of apoptotic cells by 11.4 percent. The results indicate that the ethyl acetate fraction of each plant could be used to isolate natural anticancer molecules to combat colorectal cancer.
Author (s) DetailsWamtinga Richard Sawadogo
Tang Center for Herbal Medicine Research, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637, U.S.A and Department of Anesthesia and Critical Care, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637, U.S.A and Institut de Recherche en Sciences de la Santé (IRSS/CNRST), 03 BP 7192 Ouagadougou 03, Burkina Faso.
Yun Luo
Tang Center for Herbal Medicine Research, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637, U.S.A and Department of Anesthesia and Critical Care, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637, U.S.A and Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Bethany Elkington
College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois 60612, U.S.A.
Tong-Chuan He
Department of Orthopedic Surgery and Rehabilitation Medicine, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637, U.S.A.
Chong-Zhi Wang
Tang Center for Herbal Medicine Research, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637, U.S.A and Department of Anesthesia and Critical Care, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637, U.S.A.
Chun-Su Yuan
Tang Center for Herbal Medicine Research, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637, U.S.A and Department of Anesthesia and Critical Care, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637, U.S.A.
View Book :- https://stm.bookpi.org/TIPR-V1/issue/view/38
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