An experiment was conducted to investigate the effects of
gossypol on the growth and protein content of animal cells in culture. Four
treatments examined were To = cells + media, Te = cells + ethanol, Tgl = cells
+ 5 \(\mu\)M gossypol, and TgH = cells + 20 \(\mu\)M gossypol. Total protein
was examined spectrophotometrically and the cell cultures were established
according to a standard method at Tissue Culture Laboratory Aberdeen University
Scotland United Kingdom (UK). Gossypol was obtained from Sigma, Ltd., Poole,
Dorset, UK. Secondary 3T3 animal cells and reagents standard medium were
obtained from Life Technologies Ltd. Paisley, UK. The results of this
experiment indicated that the presence of gossypol 5 \(\mu\)M and 20 \(\mu\)M
inhibited (p<0.01) the cell growth and the protein content of the animal
cells in culture. However, the cells appeared to adapt to the presence of
gossypol 5 \(\mu\)M in culture. Gossypol 20 \(\mu\)M (TgH) completely stopped
the increase in number of the cells. It was concluded that the protein content
of animal cells in culture was adversely affected by gossypol probably due to
protein-gossypol binding which reduced protein availability for growth and
protein synthesis. It is considered that gossypol may be used as an anticancer
agent.
Author(s) Details
Ismartoyo
Department of Animal Science, Hasanuddin University, Makassar, 90245,
Indonesia.
Please see the book here:- https://doi.org/10.9734/bpi/ibs/v8/1217
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