Aims: To evaluate responses to four strains of potato
spindle tuber viroid (PSTVd) and to mixed viroid/viral infection of modern
potato cultivars.
Study Design: Four PSTVd strains were used to evaluate the
tolerance of potato varieties: VP35 (LC523658), VP87 (LC523667), FP10-13
(LC523676), and NicTr-3 (LC654171). These strains were chosen based on their
different geographic origin and their different aggressiveness against a highly
susceptible tomato cv. Rutgers.
Place and Duration of Study: Laboratory of Plant Resistance
to Diseases of All-Russian Institute of Plant Protection, St. Petersburg,
between January 2022 and May 2024.
Methodology: The study included 39 modern potato cultivars
of different origin. Transcripts of four PSTVd strains were used to inoculate
tomato plants of the cv. Rutgers. Before PSTVd inoculation with tomato sap, all
potato plants were tested for viral infection by ELISA. The presence of PSTVd
in infected plants was verified by RT-PCR as well as by RT-qPCR.
Results: The strain-specificity in the response of 39 cultivars
to viroid infection was revealed. Five types of symptoms on tubers were
identified at 90 dpi. Five cultivars were identified in which, after the first
inoculation of plants with all PSTVd strains, normal in shape tubers were
formed. Average tuber weight for PSTVd- infected plants was more than twice and
a half time less compared to mock-inoculated plants (P < 0.05). All plants
of the next generation derived from infected but normally shaped tubers showed
strong symptoms of disease. After three cycles of continuous propagation in
tomato cv. Rutgers PSTVd strains became less aggressive to potato cultivars.
Mixed viroid/viral infection lead to an increase manifestation of tuber
deformation only for certain cultivars.
Conclusion: The strain specificity of the majority of
cultivars in terms of the degree of symptom development after the first
inoculation was established. Five cultivars were symptomless after the first
inoculation with four strains but were found to have severe viroid symptoms in
plants obtained from PSTVd-infected tubers. No synergism in the manifestation
of mixed viroid/viral infection symptoms on tubers was found.
Author(s) Details:
Afanasenko O. S.,
All-Russian Institute of Plant Protection, St. Petersburg, Russia.
Lashina N. M.,
All-Russian
Institute of Plant Protection, St. Petersburg, Russia.
Mironenko N. V.,
All-Russian Institute of Plant Protection, St. Petersburg, Russia.
Kyrova E. I.,
All-Russian Institute of Plant Protection, St. Petersburg, Russia.
Rogozina E. V.,
N. I. Vavilov All-Russian Institute of Plant Genetic Resources, St.
Petersburg, Russia.
Zubko N. G.,
All-Russian
Institute of Plant Protection, St. Petersburg, Russia.
Khiutti A. V.,
All-Russian Institute of Plant Protection, St. Petersburg, Russia.
Please see the link here: https://stm.bookpi.org/RACAS-V6/article/view/14148
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