The aim of this study primarily focuses on the analytical
Method Development and Validation of Triamcinolone Acetonide by using RP-HPLC
Technique. Triamcinolone acetonide is a Glucocorticosteroid with great
immunosuppressive and anti-inflammatory activity used in the treatment of
redness, itching, scaling, crusting like inflammatory skin disorders, and other
conditions like dryness with psoriasis on the skin. The proposed method is an economic and highly
sensitive, Reverse Phase High Performance Liquid Chromatographic method for
quantification of Triamcinolone acetonide is an active Pharma ingredient (API).
The efficient separation was done by using X-bridge phenyl column has a
stationary phase and a mobile phase of 50:50 v/v of acetonitrile with Octane sulphonic
acid as a buffer solution to adjust the desirable pH-2.5 condition by employing
ortho phosphoric acid. The efficient separation of Triamcinolone acetonide was
eluted at 6.22 min and its UV absorption was done at 235 nano meters at input
flow rate of 1ml/min with Photodiode Array Detector. All the results are
acceptable limits with respect to ICH guidelines and the linearity results of
Triamcinolone acetonide gave regression equation y= 43591x+14344 and
correlation coefficient is (R2) 0.9997. The % RSD is 0.24 for intra-day
precision and 0.65 for inter-day precision supports the ruggedness and the
proposed approach is eco-friendly by AGREE-Analytical GREEnness metric shows
0.72. The quantification and appropriateness of the method was studied by the various
testing parameters like recovery test, fidelity, accuracy, Robustness, and
stability of the solution, one can understand the correctness of the approach.
Author(s) Details:
Veeraswami Boddu,
Department of Chemistry, GITAM School of Science, GITAM Deemed to be
University, Visakhapatnam, PIN-530045, India.
Kasinath
Baratam,
Kasinath
Baratam, Syner-G Biopharma Group, Chennai, Tamil Nadu, India.
Please see the link here: https://stm.bookpi.org/CICMS-V8/article/view/14087
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