In two selected varieties of exotic
ornamental plants, Calathea crotalifera, an effective protocol was developed
for micropropagation. The effects of various sterilisation techniques, type of
explant, and the combination and concentration of plant growth regulators on
the induction of shoots were investigated. When treated with a combination of
30 percent (v / v) NaOCl, 70 percent (v / v ) ethanol, and 0.3 percent (w / v)
HgCl2, axillary shoot buds sprouted from rhizomes in soil-free conditions
showed a high induction rate of shoots with the lowest contamination
percentage. The highest number of multiple shoots in the MS basal medium,
supplemented with 3.5 mg / L of 6-benzylaminopurine (BAP), 1.0 mg / L of
1-naphthaleneacetic acid (NAA), 3% of sucrose and 6 g / L of plant agar for
both varieties, was obtained in the present study and was used as a
multiplication medium. When the young shoot bud explants were incised
longitudinally before subculture, microshoots were strongly induced. In order
to assess the effects of activated charcoal and L-glutamine on reducing the
issue of necrosis, chlorophyll analysis has been studied. Compared to
indolebutyric acid ( IBA), the maximum root induction on MS medium supplemented
with 1.0 mg / L 1-Naphthaleneacetic acid (NAA) was registered. The complete
regenerated plantlets were successfully acclimatised under greenhouse
conditions in the soilless medium. This is the first rapid mass propagation
study for C. All crotalifera.
Author (s) Details
Shahril Efzueni Rozali
Faculty of Science, Technology, Engineering and Mathematics, International
University of Malaya-Wales, 50480 Kuala Lumpur, Malaysia.
Kamaludin A. Rashid
Center
for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur,
Malaysia.
Rosna Mat Taha
Institute of Biological Sciences, Faculty of Science, University of
Malaya, 50603 Kuala Lumpur, Malaysia.
View Book :- https://bp.bookpi.org/index.php/bpi/catalog/book/281
No comments:
Post a Comment