Drug-drug interactions (DDIs) represent a critical
pharmacokinetic parameter that significantly influences the efficacy and safety
of therapeutic agents. While the majority of DDI studies employ in vitro
methodologies, in vivo and in silico approaches are also applied. This book
chapter provides a comprehensive summary of the various methods utilised to
study drug-drug interactions. We thoroughly explain and discuss nine prominent
in vitro techniques: UV-VIS absorption spectral analysis, conductometric analysis,
Ardon’s method, Job’s continuous variation method, Differential Scanning
Calorimetry (DSC), FTIR spectroscopic investigation, and Thin Layer
Chromatography (TLC)—all demonstrated through the detection of interactions
between two model drugs (DRUG A and DRUG B). Additionally, we cover
fluorescence spectroscopy, exemplified by its use in examining drug-protein
interactions and high-performance liquid chromatography (HPLC), as applied to
detect the interaction between drugs and their excipients. Furthermore, we
outline fundamental in vivo methods, which typically involve the use of young,
healthy animals of varying body weights. Finally, we explore three key in
silico methods: Quantitative Structure–Activity Relationship (QSAR) Models,
Molecular Docking and Molecular Dynamics (MD) Simulations, and Physiologically
Based Pharmacokinetic (PBPK) Modelling and AI and ML methods. Overall, this
chapter offers an in-depth exploration of DDI methodologies and serves as an
accessible and foundational guide for researchers wanting to conduct DDI
studies.
Author(s) Details
Fahima Aktar
Molecular Pharmacology and Herbal Drug Research Laboratory, Department of
Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000,
Bangladesh.
Farjana Akter
Department of Pharmacy, Faculty of Pharmacy, University of Dhaka, Dhaka,
Bangladesh.
Tasnuva Sharmin
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of
Dhaka, Dhaka-1000, Bangladesh.
Niaz Morshed
Department of Pharmacy, Faculty of Pharmacy, University of Dhaka, Dhaka,
Bangladesh.
Md. Shah Amran
Department of Pharmacy, School of Pharmacy and Public Health, Independent
University, Bangladesh, Bashudhara Residential Area, Plot 16, Block B,
Dhaka-1245, Bangladesh.
Please see the book here :- https://doi.org/10.9734/bpi/psnid/v9/6360
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