Introduction: Development of antibacterial agents aggravates the emerging antibiotic resistance; much attention has to be paid to natural produts. Nature has gifted with many herbs that have been found to have bactericidal and fungicidal action and these have been used from ages. One among them is Rose(rosaindica) which is easily available everywhere like other herbs. Many diseases of oral cavity are bacterial and fungal, especially all the immune compromised patients are affected with fungal and bacterial infections. Use of synthetic drugs may result in side effects and antibiotic resistance. So, the present study is all about determining the antibacterial and antifungal effect of rose. Hence, determining its application in dentistry, these can be used as an alternate to synthetic drugs to cure diseases.
Material and Methods: Oral bacteria like Streptococcus mutans and
Lactobacillus, the main microorganisms implicated in the initiation and
progression of caries, were selected for the study. Petals of Rosa indica were
used for the preparation of ethanolic extract. 100 g of the powdered sample
were soaked in 300 ml of Ethanol in a soxhlet apparatus. The sample obtained
from the soxhlet apparatus was then dissolved in (dimethyl sulfoxide) DMSO.
This is used to detect Antimicrobial activity of Rosa indica. Both zone of
inhibition and minimum inhibitory concentration (MIC) were evaluated using Agar
well diffusion method and micro-dilution method respectively. The results thus
obtained were then compared with the control against Streptococcus mutans and
Candida albicans.
Results: Zone of inhibition of rose extract was found to be 18 mm,
16 mm, 10 mm at 1000 µg, 500 µg, 250 µg
against Streptococcus mutans, which was less than the control kanamycin (24 mm)
and zone of inhibition was 26 mm, 24 mm, 22 mm at 1000 µg, 500 µg, 250 µg
against Candida albicans which was greater than the control ketaconozole (19
mm). Thus, the rose extract has both antibacterial and antifungal property.
Antifungal effect is more than antibacterial effect of rose.
Discussion: Zone of inhibition of rose extract is more when
compared to ketaconozole, an antifungal drug and less than kanamycin, an
antibiotic drug. Rose extract has both antibacterial and antifungal property.
Antifungal effect of rose is more than antibacterial effect and it is very
effective against fungal diseases.
Conclusion: We can state that roses have both antibacterial and
antifungal activity. In the present study rose extract has more antifungal
property than antibacterial property. Extract of rosaindica has promising
effect in curing fungal diseases mainly in opportunistic infections of
immunocompromised patients. This study adds knowledge encouraging the
integration of traditional herbal remedies in modern clinical practice
Author (s) Details
Swetha R
Department of Public Health Dentistry, Government Dental College and
Hospital, Kadapa, AP, India.
Shobana R
Department of Public Health Dentistry, SRM Dental College & Hospital,
Chennai, India.
Sunayana M
Department of Public Health Dentistry, SRM Dental College & Hospital,
Chennai, India.
Prabu D
Department of Public Health Dentistry, SRM Dental College & Hospital,
Chennai, India.
Raj Mohan
Department of Public Health Dentistry, SRM Dental College & Hospital,
Chennai, India.
Naveen Raj
Department of Public Health Dentistry, SRM Dental College & Hospital,
Chennai, India.
Please see the book here:-
https://doi.org/10.9734/bpi/mbrao/v3/5569
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