It is crucial to rapidly and accurately diagnose tuberculosis (TB) to reduce morbidity, mortality, and the risk of transmission. A simple, cost-effective diagnostic test to rapidly detect M. tuberculosis in clinical samples is essential. We evaluated an in-house diagnostic test based on loop-mediated isothermal amplification (LAMP) targeting the M. tuberculosis 16S rRNA gene. It was compared with the Genotype and phenotype assay for diagnosing TB in India. Out of 874 samples processed, the overall sensitivity and diagnostic accuracy were found to be 97.06% (with a confidence interval of 95.69-98.09%) and 97.03% (95.67-98.05%), respectively. The sensitivity and accuracy of the LAMP assay against the GenoType MTBDRplus and MGIT assay were 96.41% (94.61-97.73%) and 98.74% (96.36-99.74%), and 96.38% (94.61-97.69%) and 98.74% (96.38-99.74%), respectively. The kappa values were approximately 0.64 and 0.40 for the genotypic and phenotypic assays. Based on these findings, the LAMP assay could be a suitable point-of-care test for rapid diagnosis of tuberculosis in resource-limited laboratory settings.
Author
(s) Details
P.
Gunavathy
Department of Biotechnology, Indira Gandhi College of Arts and
Science, Indira Nagar, Puducherry, India.
B.
Usharani
Department of Biomedical Genetics, Institute of Basic Medical
Sciences, University of Madras, Tamil Nadu, India.
M.
Anbazhagi
Department of Environmental Science, Central University of Kerala,
Kasarcode, Kerala, India.
R.
Venkateswari
Department of Medical Biochemistry, Institute of Basic Medical
Sciences, University of Madras, Tamil Nadu,
India.
B.
Revathi Mani
Department of Biochemistry, Queen Mary’s College, Madras, Tamil
Nadu, India.
M.
Muthuraj
State TB Training and Demonstration Centre, Intermediate Reference
Laboratory, Government Hospital for
Chest Diseases, Puducherry, India.
Please see the book here:- https://doi.org/10.9734/bpi/rpmab/v6/1768
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