Sunday, 6 April 2025

Studies on Genetic Variability and Screening of Yellow Vein Mosaic Virus (YVMV) Resistance in Mutant Lines of Blackgram (Vigna mungo L. Hepper) under Field Condition | Chapter 2 | Contemporary Research and Perspectives in Biological Science Vol. 11

Background: A breeding program requires that a gene pool be made more genetically variable. In order to increase variability and find desirable recombinants, hybridisation and mutation techniques are used in tandem. The most important disease affecting and decreasing blackgram yield is yellow mosaic disease, which is caused by the Mungbean Yellow Mosaic Virus (MYMV). Blackgram crop land is being used for other cereal crop cultivation since resistant cultivars are not readily available. Breeding with these resistant cultivars is an eco-friendly and efficient way to manage MYMV in urdbean production.

Aim: The aim of the study is to assess the genetic variability and yellow vein mosaic virus (YVMV) resistance in mutant lines of blackgram (Vigna mungo L. hepper) under field condition.

Methods: In Agricultural and Horticultural Research Station, Bavikere a field experiment conducted at Kharif 2022 utilising 90 F4 M3 laid out in an Augmented design. For the summer of 2023, 30 F5 M4 were selected from the first season and laid out in an RCBD design based on seed yield, disease index, and incidence. These lines were produced by hybridising Rashmi and PU31, then treating F2 seeds at IIHR in Bangalore with gamma irradiation (20kr). Statistical analysis was performed on the data of traits means recorded on 15 selected plants for yield and its attributing traits using WINDOWSTAT version 9.2 software and R software.

Results: This study showed significant differences for most of the characteristics. The number of clusters per plant, number of pods per cluster, number of pod length, number of seeds per pod, and seed yield all showed high PCV, GCV, broad sense heritability, and genetic advancement as a percentage of mean for the first season. In terms of the number of branches per plant, number of clusters per plant, number of pods per cluster, number of pods per plant, and number of seeds per pod, high broad sense heritability with genetic advancement, moderate PCV, and GCV were showed for the second season. Estimated high PCV values suggest greater population variability in trait seed yield per plant, but the narrow range between GCV and PCV suggests that environmental influences on trait expression are less significant. The plants that exhibited the least percentage disease index and resistant reaction to YVMV were BLM 9, BLM 20, BLM 29, BLM 44, BLM 51, and BLM 58 in the first season, and BLM 30 and BLM 51 in the second.

Conclusion: This research suggests that additional crop improvement can be achieved with using these resistant high yielding mutant lines.

 

Author (s) Details

 

N. Nagamani
Department of Genetics and Plant Breeding, ZAHRS, Navile, Shivamogga, Keladi Shivvappa Nayaka University of Agricultural and Horticultural Sciences, Shivamogga, Karnataka-577204, India.

S. K. Shashikala
Department of Genetics and Plant Breeding, ZAHRS, Navile, Shivamogga, Keladi Shivvappa Nayaka University of Agricultural and Horticultural Sciences, Shivamogga, Karnataka-577204, India.

 

B. Manjunatha
Keladi Shivvappa Nayaka University of Agricultural and Horticultural Sciences, Shivamogga, Karnataka-577204, India.

 

N. Sadashiv
Department of Plantation, Spices and Aromatic Crops and Station Head, AHRS, Bavikere, Shivamogga, Karnataka-577204, India.

 

T. N. Usha
Department of Seed Technology, Directorate of Research, Keladi Shivvappa Nayaka University of Agricultural and Horticultural Sciences, Iruvakki, Shivamogga, Karnataka-577412, India.

 

Please see the book here:- https://doi.org/10.9734/bpi/crpbs/v11/4882

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