Background: Isocitrate lyase is considered as an enzymatic indicator of a cycle diagram and occupy different functions in the plant cell metabolism. Isocitrate lyase (EC 4. 1 .3. 1) catalyzes the reaction isocitrate < glyoxylate + succinate and has been reported to be widely distributed.
Objective: The objective of this study was to identify Isocitrate
lyase genes and to development the specific primers.
Methods: Research materials in this work were obtained and worked
at the State University of Varonish, Russia for the period from 2017 to 2018.
Using primers prefixes cloned of metamorphic genes Isocitratase lyase that were
able to find the presence of two genes within the gene of Amaranthus namely (icl1, icl2), which come on mentioned later in
relays products by the
polymerase chain reaction (PCR).
Results: The results of this study showed the total RNA extract
was two types of mRNA of Isocitrat lyase genes of the A. thaliana genes named
ICl2 and ICl1. The presence of two genes icl1 and ICL2 in the Amaranthus genome showed the fragment of
the ICl1 gene of arabidopsis was 53%
fragmented of the icl2 gene is 447 bp long and its homology with the icl1 gene
of arabidopsis was 36%. On the basis of sequences, specific to isocitrate lyase
genes were developed, which made it possible to investigate their expression
activityat the initial stages of Amaranthus
development.
Conclusion: The results of this study concluded that the total RNA
extract there are two types of mRNA of Isocitrate lyase genes shown in their
symmetry with A. thaliana genes icl2 and icl1.
Author
(s) Details
Saba Hadi
Department of Biology, College of Sciences, Mustansiryah University, Iraq.
Asseel M. M. Habh
Department of Biology, College of Sciences, Mustansiryah University, Iraq.
Alyaa Muhsin Yousif
Department of Biology, College of Sciences, Mustansiryah University,
Iraq.
Please see the book here:- https://doi.org/10.9734/bpi/crpbs/v11/4933
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