Picrorhiza kurroa, traditional Himalayan herb popularly
known as kutki, had been emerged now as the most promising plant for National/
International trade commodity. Excessive usages of this particular herb in
various formulations made it vulnerable. Thus, its propagation through
micro/macro propagation methods for its further better conservation has been
realized. Owing to its large demand in the local and global market for its
established pharmaceuticals and nutraceuticals potential are well recognized at
present. In view of the described fact, we access its availability from the
grower’s field from mid Himalaya of Kumaun region of Uttarakhand (India).
During rearing of accessed kutki plants from grower’s fields which were
maintained at biotech product testing facilities (green house facilities) as
well as under laboratory in vitro practices, these were nurtured and
established in pots for further in vitro establishment. Thus, pot containing
soil: sand: compost in the 1:1:1 ratio where field grown plants were nurtured
and nourished. Powdery mildew caused by the fungus stressed these plants.
However, this stress could be managed by foliar application of 0.2% (w/v)
sulfur in water and 0.2% (v/v) mild (Tween-20) aqueous detergent solutions.
Imidacloprid (0.2% v/v) was also required to spray to avoid sucking pests
(Aphids and Thripes) infestations. The data of explants after control of
infection and infestation and prior to control were compared. While
establishing explants in vitro, our data showed better establishment on infection
and infestation under control. However, molecular characterization for
infection and infestation behavior yet is underway. Murashige and Skoog medium
while supplemented with 0.5mg/L Thidiazuron (TDZ) and 0.5mg/L Indole butyric
acid (IBA) based on our previous studies and checker board for identifying
suitable concentrations of growth regulators, showedgood callus induction rate.
Callus growth was maintained through subsequent sub-culturing based on callus
growth conditions. It is worth mentioning that the use of soluble
Polyvinylpyrrolidone (PVP) 0.03-0.05% w/v significantly removed phenolics
exudates and sufficient for maintaining callus growth and viability. Beside,
orientation of molecules is important to assess their promising biological
activities. In view of this particular fact Fourier Transform Infrared (FTIR)
analysis offering valuable insights for the molecules extracted from the
induced callus for various functional groups. This will provide some
understanding of bioactivities from this important ethnic plant resource too.
Author(s) Details:
Pradip Madhukar Kharat,
Bioprospecting and Metabolomics, Molecular Biology and Genetic
Engineering, College of Basic Science and Humanities, Govind Ballabh Pant
University of Agriculture and Technology, Pantnagar, Uttarakhand, India.
Bhawna
Pandey,
Bioprospecting
and Metabolomics, Molecular Biology and Genetic Engineering, College of Basic
Science and Humanities, Govind Ballabh Pant University of Agriculture and
Technology, Pantnagar, Uttarakhand, India.
Shreya Chandola,
College of Basic Science and Humanities, Govind Ballabh Pant
University of Agriculture and Technology, Pantnagar, Uttarakhand, India.
Geeta Rawat,
College of Basic Science and Humanities, Govind Ballabh Pant
University of Agriculture and Technology, Pantnagar, Uttarakhand, India.
K C Verma,
College
of Basic Science and Humanities, Govind Ballabh Pant University of Agriculture
and Technology, Pantnagar, Uttarakhand, India.
Gurdeep
Bains,
College
of Basic Science and Humanities, Govind Ballabh Pant University of Agriculture
and Technology, Pantnagar, Uttarakhand, India.
D S Rawat,
College of Basic Science and Humanities, Govind Ballabh Pant
University of Agriculture and Technology, Pantnagar, Uttarakhand, India.
A K Gaur,
College of Basic Science and Humanities, Govind Ballabh Pant
University of Agriculture and Technology, Pantnagar, Uttarakhand, India.
Please see the link here: https://stm.bookpi.org/ACPR-V7/article/view/13542
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