Punica granatum thrives in climates with
long, hot, dry summers (90°F) and milder winters. The genus name is derived
from the Latin name Punicum malum. A 100-g serving of pomegranate seeds
contains 12% of the Daily Value (DV) for vitamin C, 16% of the DV for vitamin
K, and 10% of the DV for folate. Pomegranate seeds are high in dietary fiber
(20% DV), which is completely contained in the edible seeds. People who choose
to throw away the seeds miss out on the nutritional benefits provided by seed
fiber and micronutrients [1]. The most abundant phytochemicals in pomegranate
are the hydrolyzable tannins known as ellagitannins, which are formed when
ellagic acid and/or gallic acid bind with a carbohydrate to form pomegranate
ellagitannins [2,3], also known as punicalagins Anthocyanins are responsible
for the red color of juice. Generally, as fruit ripens, the pigmentation of the
juice increases [4]. Processing and pasteurization techniques reduce the
phenolic content of pomegranate juice [5]. The antimicrobial activity of the various
extracts (maceration with ethanol and hexane separately, and soxhlet with
ethanol) was investigated (using agar well diffusion method). Positive controls
included penicillin and ampicillin. Ethanol soxhlet extracts inhibited the
growth of all seven strains of bacteria, resulting in the detection of a zone
of inhibition. Ethanol maceration extract only had antimicrobial activity
against Neisseria gonorrhoeae, Pseudomonas aeruginosa, and Staphylococcus
aureus, whereas hexane extract did not. Using the equations obtained from the
graphs, the IC50, or half maximal (50 percent ) inhibitory concentration for
Punica granatum seed extracts, was calculated. The DPPH radical scavenging
assay was used to determine antioxidant activity. In this study, the half maximal
(50 percent) inhibitory concentration (IC50) was determined. Ethanol soxhlet
(ES) extract had the lowest IC50 value (1350.27 g/mL), hexane extract (HM) had
the highest IC50 value (3005.66 g/mL), and ethanol maceration extract (EM) had
the lowest. (4852.26 g/mL) value The following objectives were devised to
achieve the goals: Authentication of plant material The antimicrobial potential
and antioxidant activity of Punica granatum seed extracts were evaluated.
Author(s) Details
Naeem
Hasan Khan
Faculty
of Pharmacy, AIMST University, Malaysia.
Adriana Lee Tze Ying
Faculty of Pharmacy, AIMST University, Malaysia.
Candy Goo Zhi Tian
Faculty of Pharmacy, AIMST University, Malaysia.
Ooi Wei Yi
Faculty of Pharmacy, AIMST University, Malaysia.
Shantini Vijayabalan
Faculty of Pharmacy, AIMST University, Malaysia.
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