Application of Spectrophotometry in Fruit Crop Analysis | Chapter 4 | Current Research Progress in Agricultural Sciences Vol. 10

Fruit crops are essential to global agriculture and nutrition, significantly contributing to the dietary intake of vitamins, minerals, and antioxidants. The quality and nutritional content of fruits can vary widely based on factors such as variety, growing conditions, and post-harvest handling. Spectrophotometry is one of the key analytical techniques in fruit crop analysis, from measuring light absorption to assessing quality, composition, and health. Spectroscopy provides access to information about the chemical components and physical properties of fruits by obtaining optical information. This non-destructive method is essential for evaluating pigments, nutrients, phenolics, quality control, pesticide residues, and shelf-life. This study intends to evaluate the application of Spectrophotometry in fruit crop analysis. Spectrophotometry has been used for a lot of modern applications of food quality analysis and post-harvest inspection of fruits and vegetables. Recent Advances including UV-Vis, NIR, FTIR, Raman, and nanoparticle-based spectrophotometry have enhanced accuracy and sensitivity. Detailed insights into the biochemical properties of fruits have been offered by these techniques. These techniques are also aiding in the assessment of ripeness, nutritional value, and potential health benefits. Machine learning and remote sensing are expected to revolutionize the field by improving the accuracy of disease diagnosis and quality assessment. Additionally, portable and handheld spectrophotometers are becoming increasingly popular, allowing for on-site analysis in orchards and farms. This accessibility enables real-time decision-making, empowering farmers to implement precise agriculture practices and improve crop management. The future prospects of spectrophotometry in fruit crop analysis are promising, with ongoing research and development focusing on enhancing its capabilities through integration with emerging technologies.

 

Author (s) Details

 

Nishchala Arya
Department of Fruit Science, College of Horticulture, Dr. Yashwant Singh Parmar University of Horticulture and Forestry,Nauni-Solan 173 230, India.

 

Shiv Kumar Shivandu
Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India.

 

Ishani Sharma
Department of Fruit Science, College of Horticulture, Dr. Yashwant Singh Parmar University of Horticulture and Forestry,Nauni-Solan 173 230, India.

 

Gaurav Rana
Department of Microbiology, Career Point University, Bhoranj, Hamirpur 176 041, India

 

Rolika Gupta
Department of Microbiology, Career Point University, Bhoranj, Hamirpur 176 041, India

 

Akriti Banya
Department of Fruit Science, College of Horticulture, Dr. Yashwant Singh Parmar University of Horticulture and Forestry,Nauni-Solan 173 230, India.

 

Please see the book here:- https://doi.org/10.9734/bpi/crpas/v10/4686

Quantitative Determination of an Antiviral Drug Ganciclovir by Redox-based Spectrophotometric Method | Chapter 11 | Pharmaceutical Research - Recent Advances and Trends Vol. 1

 

Ganciclovir functions as an antiviral medication crucial for averting cytomegalovirus disease among recipients of bone marrow and solid organ transplants. Furthermore, it plays a pivotal role in managing individuals with compromised immune systems by combatting viral infections effectively. This study aims to develop and validate a simple, rapid and sensitive redox-based spectrophotometric method for the quantification of Ganciclovir in pure and pharmaceutical dosage forms. The development process involved utilizing 0.1 M HCl as the solvent along with a combination of ferric chloride and 1,10-phenanthroline as the chromogenic reagent.

After development, the method underwent optimization across various conditions to ensure its efficacy, followed by rigorous statistical validation. Based on the optimization studies, it was determined that the optimal composition for the method included a mixture containing 0.3% w/v of ferric chloride and 0.5% w/v of 1,10-phenanthroline, with a drug-to-reagent mole ratio of 0.5:0.5. When measured at 510.0 nm, the method exhibited linearity within the concentration range of 5.0-30.0 μg/mL for Ganciclovir. Demonstrating its sensitivity, the method showcased a low limit of detection and quantification values, recorded at 0.30 and 0.90 μg/mL, respectively. The validation parameters of the method were found to align with the standards outlined by the ICH guidelines. With a % assay value of 99.2, the method demonstrated its suitability for pharmaceutical formulation analysis. The simplicity of the developed method renders it suitable for application in quality control testing of Ganciclovir within formulations.

 

Author(s) Details:

 

Dr. Naraparaju Swathi, M. Pharm., Ph.D.
Department of Pharmaceutical Chemistry, Gokaraju Rangaraju College of Pharmacy, Hyderabad-500090, Telangana, India.

 

Mrs. Barla Karuna Devi, M. Pharm. (Ph.D.)
Department of Pharmaceutical Chemistry, Gokaraju Rangaraju College of Pharmacy, Hyderabad-500090, Telangana, India.

 

Dr. Anumolu Durga Pani Kumar
Department of Pharmaceutical Analysis, Gokaraju Rangaraju College of Pharmacy, Hyderabad-500090, Telangana, India.

 

Please see the book here:  https://doi.org/10.9734/bpi/prrat/v1/196

Determination of Vanadium (V) Using Metanil Yellow Dye as an Analytical Reagent: Spectrophotometric Analysis | Chapter 2 | Advanced Concepts in Pharmaceutical Research Vol. 9

 

Vanadium finds applications in various industries, accurate determination of vanadium levels ensures quality control and monitoring vanadium levels in drinking water and air helps in public health. The development of spectrophotometric determination using Metanil yellow dye contributes to the advancement of analytical chemistry. The method quantifies trace levels of vanadium in various samples and serves diverse societal needs, including environmental protection, industrial quality control, public health, scientific advancement, regulatory compliance, and resource management. A simple, rapid, selective and sensitive method for the spectrophotometric determination of microgram amount of Vanadium (V) utilizing the reaction of Metanil yellow dye in 40% Ethyl Alcohol medium. Metanil yellow reacts with Vanadium (V) instantaneously at room temperature in a slightly acidic medium. The complex exhibits an absorption maximum at 512 nm of UV- visible spectrophotometer with a molar absorptivity of 2.21 × 105 litre mol-1 cm-1. The sensitivity is 4.52 n gm of Vanadium ml-1 and the optimum range for the effective spectrophotometric determination is 0.05-0.41µg ml-1. A five-fold molar excess of the dye is necessary for the full development of the colour intensity.  The influence of some common ions is reported.

Author(s) Details:

Aparna Somendra Bhardwaj,
Department of Chemistry, Mithibai College, Vile Parle (W), Mumbai, 400056, Maharashtra, India.



Please see the link here: https://stm.bookpi.org/ACPR-V9/article/view/14361

Quality Evaluation of Selected Drug in Pharmaceutical Formulations by Spectrophotometric Estimation of Imatinib Mesylate in Pure and Tablet Dosage Form| Chapter 7 | Current Overview on Pharmaceutical Science Vol. 1

 For the decision of imatinib mesylate in both allure pure and tablet measure forms, a simple and impressionable spectrophotometric approach has been settled. Imatinib mesylate is a white crystalline powder. The submitted approach is founded on the calculation of ultraviolet light absorption in water purified by distillation. The UV spectrum of Imatinib mesylate in distilled water displayed λmax at 256 nm. Beer’s standard is valid in the aggregation range of 2-12 μg/ml. In terms of precision, veracity, ruggedness, and strength, this method has been corroborated. The procedure is reproducible and discriminating for the estimation of the likely drug, according to statistical reasoning.

Author(s) Details:

Kumar Raja Jayavarapu,
Department of Pharmaceutical Analysis, Mother Teresa Pharmacy College, Sathupally-507303, India.

V. D. Sundar,
Research Labs, GIET School of Pharmacy, Rajahmundry-533294, AP, India.

Please see the link here: https://stm.bookpi.org/COPS-V1/article/view/8966

Metformin HCL: Visible Spectrophotometric Determination in Pure and Dosage Forms| Chapter 8 | Progress in Chemical Science Research Vol. 1

Metformin HCl is an antidiabetic medication that belongs to the biguanidine pharmacological family. Metformin HCl (MET) in pure form and pharmaceutical formulations is determined using simple and innovative visible spectrophotometric procedures. The production of coloured complexes allows for the development of selective visible spectrophotometric approaches. The method is based on the reaction between the medication and metal ions such as Co(II) sulphate and Cu(II) chloride. The highest absorption wavelength for Co(II)-MET complex is 518 nm, while Cu(II)-MET complex is 660 nm. To get the maximum absorbance, the optimal conditions for complex formation are examined and optimised. The findings are compared statistically in terms of accuracy and precision using student's t and F tests with a 95% confidence level. The findings enable the use of established technologies for MET analysis in both pure form and commercial tablets.

Author(s) Details:

Kumble Divya,
Department of Chemistry, B.M.S College for women (Autonomous), Basavanagudi, Bengaluru-560 004, Karnataka, India.

Please see the link here: https://stm.bookpi.org/PCSR-V1/article/view/6766

Recent Study on Development and Validation of Loperamide Hydrochloride Tablet Analysis Method with Absorbance and Area under Curve Methods Spectrophotometrically | Chapter 6 | Technological Innovation in Pharmaceutical Research Vol. 3

 The absorbance method and the area under the curve method were used to develop and validate the loperamide hydrochloride tablet method of analysis. The optimum solvent in this study was 0.1 N methanol and hydrochloric acid (9:1), with maximum absorption at wavelength 259.00 nm.

The linearity of loperamide hydrochloride was determined in the concentration range 200-600 ppm using the absorbance method and the areas under the curve method, with correlation coefficient values of 0.9998 and 0.9865, respectively. The findings revealed that loperamide hydrochloride levels in The absorbance method and the area under the curve method yielded 105.71 percent and 96.20 percent generic pills, respectively. The findings of the absorbance method and the area under the curve method for determining loperamide levels in trademark pills were 102.85 percent and 98.57 percent, respectively. Both samples met the requirements of Pharmacopoeia Indonesia edition V, which are 90% to 110 percent. The absorbance method for the analysis of loperamide hydrochloride in tablets is legitimate, however the area under the curve technique is unsuitable for the analysis of loperamide hydrochloride in tablets.

Author (s) Details

Harrizul Rivai
Faculty of Pharmacy, Andalas University, Indonesia.

Renny Puspita
School of Pharmaceutical Science, Kurao, Indonesia.

Sesry Misfadhila
School of Pharmaceutical Science, Kurao, Indonesia.

View Book : - https://stm.bookpi.org/TIPR-V3/article/view/1149

The Use of Spectrophotometric and Thermodynamic Methods for the Determination of Glibenclamide | Chapter 8 | Current Research and Development in Chemistry Vol. 4

 

For the assay of glibenclamide, a spectrophotometric and thermodynamic method is defined. The technique involves a glibenclamide charge transfer complexion reaction with 2,3-Dichloro-5,6-Dicyano-1,4-benzoquinone (DDQ) to form a very intense coloured charge transfer complex with a 1:1 stoichiometric ratio. Under tailored experimental conditions, the resulting coloured product was analysed at 474 nm. Beer-Lambert 's law was obeyed over the 20-100 μg / ml concentration range. The apparent absorptivity of the molar was measured and 0.99 ⁇ 103 L.mol- 1 cm-1 was found. The corresponding sensitivity of Sandell, detection limits, and quantification were stated. The thermodynamic parameters have been defined as well. The proposed method was applied successfully to the determination of glibenclamide in pure and commercial forms with strong average recovery of 98.24 percent. The student t-test and f-test were used for statistical comparison at a 95 percent confidence level.

Author (s) Details

Nwanisobi Gloria
Department of Pure and Industrial Chemistry, Faculty of Physical Sciences, Nnamdi Azikiwe University, Awka, Nigeria.

Ukoha Pius
Department of Pure and Industrial Chemistry, Faculty of Physical Sciences, University of Nigeria, Nsukka, Nigeria.

View Book :- https://bp.bookpi.org/index.php/bpi/catalog/book/282