Hyperglycemic Control of Moringa oleifera Leaves Aqueous Extract and Glibenclamide Tablets in Alloxan Monohydrate Induced Diabetic Rats: A Comparative Analysis | Chapter 3 | Current Overview on Pharmaceutical Science Vol. 9

 This division aims to compare the hyperglycemic control of M. oleifera leaves liquid extract and Glibenclamide tablet in alloxan monohydrate inferred diabetic rats.In this experiment, Twenty-four female Wister albino rats, fashioned diabetic using alloxan monohydrate, taken either M. oleifera extract, glibenclamide or distilled water were delivered intragastric.The verdicts showed that Rats that taken distilled water had a mean abstaining blood sugar of 329.3+44.9mg/dl at first, which raised to 448.0+189.9mg/dl on day 14, all   the rats were dead by era 21. The rats that received M. oleifera had glucose 443.4+134.7mg/dl at the beginning, discontinued to 166.5+162.79mg/dl by day 14, and to 88.7+41.0 mg/dl by epoch 28. Rats that received glibenclamide had blood sugar 517.6+139.3mg/dl at first, dropped to 209.0+201.9mg/dl on day14, and to 89.7+42.85mg/dl eventually 28.  The blood sugar levels in the M.oleifera and glibenclamide groups restored to normal by era 21 and remained inside the normal range as far as the experiment was completed eventually 28. Based on the findings concerning this study, it can be concluded that Moringa oleifera leaves liquid extract has similar pattern to glibenclamide pellet in causing hypoglycemia to alloxan monohydrate persuaded diabetic rats.

Author(s) Details:

Josephine N. Kasolo,
Department of Physiology, Makerere College of Health Sciences, Uganda.

Agnes Namaganda,
Department of Physiology, Makerere College of Health Sciences, Uganda.

Joshua Nfambi,
Department of Physiology, Makerere College of Health Sciences, Uganda.

Ivan Kimuli,
Department of Physiology, Makerere College of Health Sciences, Uganda.

Haruna Muwonge,
Department of Physiology, Makerere College of Health Sciences, Uganda.

Allan Lugaajju,
Department of Physiology, Makerere College of Health Sciences, Uganda.

Isaac Okullo,
Department of Dental Surgery, Makerere College of Health Sciences, Uganda.

Please see the link here: https://stm.bookpi.org/COPS-V9/article/view/10168

Pharmacogenetics of Allelic Variants of CYP2C9 and Clinical Implications among Mexican Patients with Diabetes Mellitus Type 2 Undergoing Treatment with Glibenclamide and Metformin | Chapter 7 | Current Overview on Pharmaceutical Science Vol. 7

 Diabetes  Mellitus Type 2 (DMT2) is a important public health concern in Mexico as well as everywhere.  The majority of Mexican cases with diabetes mellitus type 2 (DMT2) (67.9-85.0%) are prescribed sulphonylureas (SUs), that are metabolized by cytochrome P450 2C9. The polymorphisms of CYP2C9 are thought to be being the reason for the extensive variability about SU responses. The current member aims todescribe CYP2C9 polymorphisms (*2, *3 and IVS8-109T) within a sample of Mexican patients accompanying DMT2, while suggesting the potential clinical implications in conditions of glibenclamide response instability. The study included 248 patients accompanying DMT2 who initially agreed to be studied, those contained in the study were treated accompanying glibenclamide (n=11), glibenclamide combined with metformin (n=112) or metformin (n=76), and were afterward genotyped using a reverse transcription all-inclusive polymerase chain reaction (PCR), endpoint allelic bias and PCR amplifying enzymatic restriction fragment long variety. Glibenclamide significantly reduced the pre?prandial sweet liquid (P<0.01) and the percentage of glycated red body fluid (%HbA1c; P<0.01) for IVS8-109A>T compared with linked glibenclamide and metformin treatment. Concerning the various situations with respect to the different genotypes, the percentages acquired were as follows: Glibenclamide A/A, HbA1c<6.5=33.3%; glibenclamide + metformin A/A, HbA1c<6.5=24.6%; gliben-clamide A/T, HbA1c<6.5=33.3%; glibenclamide + metformin A/T, HbA1c<6.5=25%; glibenclamide T/T, HbA1c<6.5=100%; and glibenclamide + metformin T/T, HbA1c<6.5=12.5%. Overall, these judgments show that, while genetically customised prescriptions remain a good goal for increasing healing success, neither allelic modifications nor dosages evidenced a strong equating with biomarker levels within the intentional population; thus, more exact experimental and practical investigations are needed.

Author(s) Details:

Patricia Cuautle Rodríguez,
Department of Pharmacology, School of Medicine, National University of Mexico, Mexico City, Mexico.

Nidia Samara Rodríguez-Rivera,
Departamento de Farmacología, Facultad de Medicina, UNAM, Mexico City, Mexico.

Fernando de Andrés Segura,
Department of Analytical Chemistry and Food Technology, Faculty of Pharmacy, University of Castilla-La Mancha, Albacete, Spain.

Adrián LLerena,
Institute of Biosanitary Research of Extremadura and of the Clinical Research Center of the University Hospital of Badajoz, Spain.

Fernando Castillo-Nájera,
Centro de Salud T-III Portales, Servicios de Salud, Gobierno de la Ciudad de México, México.

Juan Molina-Guarneros,
Departamento de Farmacología, Facultad de Medicina, UNAM, Mexico City, Mexico.

Please see the link here: https://stm.bookpi.org/COPS-V7/article/view/9699

The Use of Spectrophotometric and Thermodynamic Methods for the Determination of Glibenclamide | Chapter 8 | Current Research and Development in Chemistry Vol. 4

 

For the assay of glibenclamide, a spectrophotometric and thermodynamic method is defined. The technique involves a glibenclamide charge transfer complexion reaction with 2,3-Dichloro-5,6-Dicyano-1,4-benzoquinone (DDQ) to form a very intense coloured charge transfer complex with a 1:1 stoichiometric ratio. Under tailored experimental conditions, the resulting coloured product was analysed at 474 nm. Beer-Lambert 's law was obeyed over the 20-100 μg / ml concentration range. The apparent absorptivity of the molar was measured and 0.99 ⁇ 103 L.mol- 1 cm-1 was found. The corresponding sensitivity of Sandell, detection limits, and quantification were stated. The thermodynamic parameters have been defined as well. The proposed method was applied successfully to the determination of glibenclamide in pure and commercial forms with strong average recovery of 98.24 percent. The student t-test and f-test were used for statistical comparison at a 95 percent confidence level.

Author (s) Details

Nwanisobi Gloria
Department of Pure and Industrial Chemistry, Faculty of Physical Sciences, Nnamdi Azikiwe University, Awka, Nigeria.

Ukoha Pius
Department of Pure and Industrial Chemistry, Faculty of Physical Sciences, University of Nigeria, Nsukka, Nigeria.

View Book :- https://bp.bookpi.org/index.php/bpi/catalog/book/282