Formulation and Antibacterial Assessment of a Herbal Cream Containing Biophytum sensitivum D.C.| Chapter 1 | Pharmaceutical Science: New Insights and Developments Vol. 8

 

Aim: Biophytum sensitivum DC Oxalidaceae is a traditional medicinal plant for treating bacterial infections. The goal of the current study was to create and assess an antibacterial cream of hydroalcoholic extract from Biophytum sensitivium DC.

 

Materials and Methods: This plant was extracted using the hydroalcoholic method and further evaluated for antibacterial activity against Escherichia coli (E. coli) by agar well diffusion method.

 

Results: The presence of phenols and flavonoids in the extract of Biophytum sensitivum DC Oxalidaceae exhibited significant activity on E. coli organism and activity which is compatible with standard antibiotics.

 

Conclusion: The chemical constituents were tested to identify phenols and flavonoids. The use of hydroalcoholic extract resulted in antibacterial activity, and an antibacterial cream was further formulated.

 

 

Author(s) Details

Haripriya G
Department of Pharmacognosy, JSS College of Pharmacy, Mysuru, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.

 

Krithika R
Department of Pharmacognosy, JSS College of Pharmacy, Mysuru, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.

 

LisMaria Joseph
Department of Pharmacognosy, JSS College of Pharmacy, Mysuru, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.

 

Suresh J
Department of Pharmacognosy, JSS College of Pharmacy, Mysuru, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.

 

Please see the book here:- https://doi.org/10.9734/bpi/psnid/v8/6111

Analysis of Gallic Acid and Kaempferol in Chloroform and Ethanol Extract of Cassia hirsuta Seeds by High-Performance Liquid Chromatography | Chapter 5 | Pharmaceutical Research: Recent Advances and Trends Vol. 5

Background: Cassia hirsuta plant belongs to the family Caesalpiniaceae and is commonly called stinking cassia and hairy senna. It is an alternative to coffee and is also used for stomach troubles, dysentery, abscesses, rheumatism, fever, and other diseases. High-performance liquid chromatography (HPLC) with spectrophotometric detection has been widely used for the identification and quantification of phenolics and flavonoids in plant extracts.

 

Aim: The present study's aim was to analyze the gallic acid and kaempferol in chloroform and ethanol extract of Cassia hirsuta seeds by high-performance liquid chromatography (HPLC) method.

 

Materials and Methods: The plant was collected from Bagalkot, Karnataka, India. In this study, the HPLC analysis of gallic acid and kaempferol was done by selected HPLC methods and these were validated for linearity, precision, limit of detection, limit of quantitation, repeatability, and reproducibility. The corresponding concentration of gallic acid and kaempferol against respective peak area values was determined using the gallic acid and kaempferol calibration curves, respectively.

 

Results: A calibration curve was established for gallic acid and kaempferol by injecting 4, 8, 12, 16, 20, and 24 µg/mL of standard solution. In the results, it was found that the linearity was good with regression equations y=36934x-10162 and y=61050x-9395.5 for gallic acid and kaempferol, respectively, and the correlation coefficient (R2) was 0.9982 and 0.9992, respectively. Percentage relative standard deviation of 1.6% and 0.71% for gallic acid and kaempferol, respectively, indicates the high precision. Ethanol extract of C. hirsuta shows a high content of both gallic acid and kaempferol as compared to chloroform extract of C. hirsuta.

 

Conclusion: In the present study, gallic acid and kaempferol content were determined in ethanol and chloroform extract of C. hirsuta. The developed HPLC methods have enabled rapid, linear, accurate, and reproducible analysis in these two extracts of C. hirsuta. The developed method can be used for quantitative analysis and quality control of extracts and commercial samples of other species containing gallic acid and kaempferol.

 

Author(s) Details

Mallappa H. Shalavadi
Department of Pharmacology, BVVS’s Hanagal Shri Kumareshwar College of Pharmacy, Bagalkot, Karnataka, India.

 

V. M. Chandrashekhar
Department of Pharmacology, BVVS’s Hanagal Shri Kumareshwar College of Pharmacy, Bagalkot, Karnataka, India.

 

I. S. Muchchandi
Department of Pharmacology, BVVS’s Hanagal Shri Kumareshwar College of Pharmacy, Bagalkot, Karnataka, India.

 

Please see the book here:-https://doi.org/10.9734/bpi/prrat/v5/1309

Assessment of The Impact of Biological Products on the Quality of Potato Tubers | Chapter 6 | Emerging Issues in Agricultural Sciences Vol. 7

 The aim of the study search out assess the effect of advantageous microorganisms introduced into the soil on picked indicators of the feature of edible vegetable tubers. The study evaluated an early, honest, drought-resistant, extreme cold-resistant and stress-opposing potato type of the Russian selection "Udacha". When increasing potatoes, organic products "Organic", "Protection of ancestries" and "Crop rotation" were secondhand, which involve bacteria and fungi in allure composition. The experiment help 4 options: 1) Control, 2) "Organic", 3) "Protection of roots", 4) "Crop turn". Biological products "Organic" and "Protection of ancestries" were applied when setting potatoes, and the organic product "Crop turn" was applied two weeks before establishiing. In the grown vegetable tubers, the starch arrangement was determined utilizing the polarimetric method, the source of nourishment C composition was determined utilizing the visual titration design, the polyphenol composition was driven using the spectrophotometric design applying Folin's acid-base indicator, the antioxidant activity was driven using potassium permanganate titration, and the nitrate arrangement was determined utilizing an ion-selective terminal on an ion meter. The results of the research demonstrated that in potato tubers the vigor composition was the lowest in the control variant (14.9%), and the topmost in the variant with the organic product “Crop Rotation” (17.8%); the arrangement of vitamin C was rude in the control variant (18.9 mg/100 g), and it was the highest in the variant accompanying the biological device “Crop Rotation” (25.3 mg/100 g); the composition of polyphenols (in mg-eq of gallic acid per g of dry burden) was the highest in the variant accompanying the biological produce “Organic” (4.1), and the lowest in the control variant (3.0); antioxidant exercise (in mg-eq of gallic acid per g of dry weight)  was the lowest in the control variant (6.5), and it was the capital in the variants accompanying biological production "Organic" and "Crop rotation" (8.4); the arrangement of nitrates decreased completely experimental modifications in comparison accompanying the control variant. The application of advantageous bacteria and fungi into the soil considerably contributed to the bettering of the quality signs of grown potato tubers.

Author(s) Details:

S. P. Zamana,
State University of Land Use Planning, 15 Kazakova St., Moscow, 105064, Russia.

T. V. Papaskiri,
State University of Land Use Planning, 15 Kazakova St., Moscow, 105064, Russia.

T. D. Kondratieva,
Bioelements limited Office G56, The Bio Centre, Innovation Way, York Science Park, YO10 5NY, United Kingdom.

D. A. Shapovalov,
State University of Land Use Planning, 15 Kazakova St., Moscow, 105064, Russia.

T. G. Fedorovsky,
State University of Land Use Planning, 15 Kazakova St., Moscow, 105064, Russia.

Please see the link here: https://stm.bookpi.org/EIAS-V7/article/view/11851

Biochemical and Physiological Alteration in Annual Moringa Cv. PKM-1 Leaves Impacted by Termination of Apical Growth | Chapter 6 | Research Highlights in Agricultural Sciences Vol. 9

 A trial was transported to evaluate the physical and biochemical changes in M. oleifera (L.) Millsp. cv. PKM 1 persuaded by terminating sharp progress by way of course back and stingy situations. After the new dash arose from title back and trimming situations superior to flower introduction, the connotations of total phenol, dissolved protein, nitrate reductase, and chlorophyll were calculated. Chlorophyll, nitrate reductase, protein, and phenol content in leaves were powerfully affected by altitude and the point at that sharp development was blocked. Except for dissolved protein, that was bred by title back at 30 cm, the corporeal and biochemical limits were considerably impressed by course back at 70 cm linked accompanying stingy 100 days following in position or time course back.  Disturbing the sharp tumor leads to sideways arm rise, this process delays the progress in addition to dry matter disposal. Accumulation of foods in stem and leaves improves the digestive characteristic of leaves. This plain method concede possibility be used to exploit the nutraceutical characteristics of moringa Cv. PKM-1 leaves.

Author(s) Details:

V. Divyabharathi,
Department of Horticulture, Institute of Agriculture, TNAU, Tiruchirappalli, India.

V. Swaminathan,
Department of Horticulture, Retd., AC & RI, TNAU, Madurai, India.

P. Paramaguru,
Department of Horticulture, HC & RIW, TNAU, Tiruchirappalli, India.

K. Venkatesan,
Department of Crop Physiology, HC & RI, TNAU, Periyakulam, Theni, India.

T. Anitha,
Department of Biochemistry, HC & RI, TNAU, Periyakulam, Theni, India.

Please see the link here: https://stm.bookpi.org/RHAS-V9/article/view/9472