Analysis of Gallic Acid and Kaempferol in Chloroform and Ethanol Extract of Cassia hirsuta Seeds by High-Performance Liquid Chromatography | Chapter 5 | Pharmaceutical Research: Recent Advances and Trends Vol. 5

Background: Cassia hirsuta plant belongs to the family Caesalpiniaceae and is commonly called stinking cassia and hairy senna. It is an alternative to coffee and is also used for stomach troubles, dysentery, abscesses, rheumatism, fever, and other diseases. High-performance liquid chromatography (HPLC) with spectrophotometric detection has been widely used for the identification and quantification of phenolics and flavonoids in plant extracts.

 

Aim: The present study's aim was to analyze the gallic acid and kaempferol in chloroform and ethanol extract of Cassia hirsuta seeds by high-performance liquid chromatography (HPLC) method.

 

Materials and Methods: The plant was collected from Bagalkot, Karnataka, India. In this study, the HPLC analysis of gallic acid and kaempferol was done by selected HPLC methods and these were validated for linearity, precision, limit of detection, limit of quantitation, repeatability, and reproducibility. The corresponding concentration of gallic acid and kaempferol against respective peak area values was determined using the gallic acid and kaempferol calibration curves, respectively.

 

Results: A calibration curve was established for gallic acid and kaempferol by injecting 4, 8, 12, 16, 20, and 24 µg/mL of standard solution. In the results, it was found that the linearity was good with regression equations y=36934x-10162 and y=61050x-9395.5 for gallic acid and kaempferol, respectively, and the correlation coefficient (R2) was 0.9982 and 0.9992, respectively. Percentage relative standard deviation of 1.6% and 0.71% for gallic acid and kaempferol, respectively, indicates the high precision. Ethanol extract of C. hirsuta shows a high content of both gallic acid and kaempferol as compared to chloroform extract of C. hirsuta.

 

Conclusion: In the present study, gallic acid and kaempferol content were determined in ethanol and chloroform extract of C. hirsuta. The developed HPLC methods have enabled rapid, linear, accurate, and reproducible analysis in these two extracts of C. hirsuta. The developed method can be used for quantitative analysis and quality control of extracts and commercial samples of other species containing gallic acid and kaempferol.

 

Author(s) Details

Mallappa H. Shalavadi
Department of Pharmacology, BVVS’s Hanagal Shri Kumareshwar College of Pharmacy, Bagalkot, Karnataka, India.

 

V. M. Chandrashekhar
Department of Pharmacology, BVVS’s Hanagal Shri Kumareshwar College of Pharmacy, Bagalkot, Karnataka, India.

 

I. S. Muchchandi
Department of Pharmacology, BVVS’s Hanagal Shri Kumareshwar College of Pharmacy, Bagalkot, Karnataka, India.

 

Please see the book here:-https://doi.org/10.9734/bpi/prrat/v5/1309

Analytical Method Validation Parameters (USP/ICH) | Chapter 1 | Novel Aspects on Chemistry and Biochemistry Vol. 9

Chemical data has been generated by placing validation into the technique. In an attempt to prevent their misguided use and ensure scientific correctness and consistency among publications, analytical method validation, thinking about the maximum relevant processes for checking the best parameters of analytical methods, and using numerous relevant overall performance indicators, including selectivity, specificity, accuracy, precision, linearity, range, limit of detection (LOD), limit of quantification (LOQ), ruggedness, and robustness, are heavily discussed.

Author(s) Details:

V. Siddaiah,
Department of Chemistry, Andhra University, Visakhapatnam-530003, India.

Please see the link here: https://stm.bookpi.org/NACB-V9/article/view/13026

UV and RP-HPLC Method Development and Validation for Estimation of Cilnidipine| Chapter 7 | Current Overview on Pharmaceutical Science Vol. 3

 The current experiment was devised to develop and endorse a simple RP-HPLC methods for determining Cilnidipine. Cilnidipine was quantified utilizing UV spectroscopy. Action separation of Cilnidipine was realized by using a C18 pillar. Mobile phase blend of methanol: water (90:10 v/v) was pressure at the flow of 1 ml/min. Detection At 241 nm, detection was completed activity. In accordance with the International Conference on Harmonization's (ICH) Q2R1 recommendations, confirmation parameters were determined. With a parametric value (r2) of 0.999, the uniformity curve for cilnidipine was linear over the range of 2–10 mg/ml.  The plan was noticed to be correct (101.66% recovery), exact (intraday, 1.65 and inter epoch, 1.38) and robust (% RSD was premeditated to be 0.66, 0.58 and 0.81 for alternative in mobile phase arrangement, wave length and flow speed respectively) for the reasoning of Cilnidipine. The developed system has successfully achieved all validation tests and can be used to estimate the attendance of cilnidipine in both drug formulations and bulk.

Author(s) Details:

Biswajit Samantaray,
School of Pharmacy and Life Sciences, Centurion University of Technology and Management, Odisha, India.

Satish Kanhar,
School of Pharmacy and Life Sciences, Centurion University of Technology and Management, Odisha, India.

Ashirbad Nanda,
School of Pharmacy and Life Sciences, Centurion University of Technology and Management, Odisha, India.

Please see the link here: https://stm.bookpi.org/COPS-V3/article/view/9112

Developing an Analytical Method and Validation for Estimation of Residual Solvents in Gliclazide Using Gas Chromatography| Chapter 4 | Challenges and Advances in Pharmaceutical Research Vol. 9

 The current work discusses developing a novel method for estimating residual solvents in gliclazide using gas chromatography and determining its consistency, reliability, and reproducibility through validation. A Gas chromatograph equipped with a flame ionization detector, column DB-624 (60 m x 0.32 mm x 1.8 µm) with Nitrogen as carrier gas was used and the column temperature was 40°C (hold for 15 mins) and increased to 240°C at 20°C per min. According to the protocol, the solutions were made using N-Methyl-2-Pyrrolidone (NMP) diluent and suitably administered in the order specified. System appropriateness, Specificity, Linearity and Range, Accuracy, Precision, Limit of Detection, Limit of Quantitation, Ruggedness and Robustness were the validation parameters examined. Each validation parameter's test data has been compiled and documented. It was discovered that each parameter's results were combined with the specified acceptance criteria. As a result, the established method was regarded as reproducible, reliable, and consistent. The analysis method was determined to be dependable, consistent, and reproducible. It conforms with all parameters examined.

Author(s) Details:

Burhanuddin Madriwala,
Department of Pharmaceutical Chemistry, M.S. Ramaiah University of Applied Sciences, Bengaluru - 560054, Karnataka, India.

Judy Jays,
Department of Pharmaceutical Chemistry, M.S. Ramaiah University of Applied Sciences, Bengaluru - 560054, Karnataka, India.

Please see the link here: https://stm.bookpi.org/CAPR-V9/article/view/8673

Determination of Radiofrequency in ENT| Chapter 14 | Current Overview on Disease and Health Research Vol. 3

 The current review was expected to evaluate the viability of Radiofrequency as a device for systems for wheezing - rest apnoea. The boundaries surveyed were: postoperative torment scores, intra and postoperative blood misfortune, decrease in abstract wheezing sounds by understanding and accomplice and decrease in AHI postoperatively. A powerful treatment for wheezing and obstructive rest apnoea is radiofrequenc removal techniques.IIt has various advantages, including as exact entry points, generally bloodless field when utilized properly, decreased postoperative agony, and amazing mending with fibrosis, which assists with hardening tissues.

Author(s) Details:

N. Shah,
St.Elizabeth, Dalvi and Cumballa Hill hospitals Mumbai, India.

Please see the link here: https://stm.bookpi.org/CODHR-V3/article/view/8060

Recent Study on a High-Precision Registration Technology Based on Bundle Adjustment in Structured Light Scanning System | Chapter 12 | Theory and Practice of Mathematics and Computer Science Vol.2

 

In this paper, in order to address this problem, we propose a high-precision registration method based on multiple view geometry theory. In the areas of reverse engineering, relic preservation, biomedicine, the most powerful methods of obtaining three-dimensional (3D) surface data of objects, organised light scanning device based on binocular stereo vision, have been commonly used. With the rising number of registrations when measuring a large scale object using standardised light scanning, the multiview 3D data registration accuracy will decrease. First, during the scanning process, a multiview network is built. To refine the multiview network to achieve high-precision global control points, the package adjustment system of optical near range photogrammetry is used. After that, the 3D data is documented with the global control points under each local coordinate of each scan. In the conventional registration procedure, the approach overcomes the accumulation of errors and decreases the time consumption of the following 3D data global optimization. The accuracy and reliability of multiview 3D scan registration is improved. The effectiveness of the proposed algorithm will be tested by experiments.

Author (s) Details

Jianying Yuan
Chengdu University of Information Technology, Chengdu 610225, China.

Qiong Wang
College of Mechanical Engineering, Southwest Jiaotong University, Chengdu 610031, China.

Xiaoliang Jiang
Quzhou University, Quzhou 324000, China.

Bailin Li
College of Mechanical Engineering, Southwest Jiaotong University, Chengdu 610031, China.

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