Method Development and Validation for the Estimation of Bortezomib in Pure and Its Pharmaceutical Tablet Dosage form by UV Spectroscopy | Chapter 13 | Novel Aspects on Pharmaceutical Research Vol. 1

 Background: Bortezomib is an antagonistic-cancer cure used to treat multiple myeloma and mantle container lymphoma.Methods: The UV Spectroscopy method for reasoning of bortezomib was developed and legitimized as per ICH guidelines. The estimation of bortezomib in the clean and tablet portion of drug or other consumable form was carried out at the maximum absorbance at 365 nm.The Results: The order was found to be uninterrupted and obeys stout law in the aggregation range 5-25µg/ml with a correlation cooperative 0.999, the developed means was validated as per ICH directions and was found to be correct and precise.Conclusion: A accelerated novel precise and correct UV Spectroscopy method was grown and validated and can be secondhand for regular study for the estimation of bortezomib.

Author(s) Details:

R. Nageswara Rao,
Department of Pharmaceutical Analysis, Santhiram College of Pharmacy, Nandyal-518501, Andhra Pradesh, India.

V. Ravikumar,
Department of Pharmaceutical Biotechnology, School of Pharmacy, Guru Nanak Institutions Technical Campus , Hyderabad, Telangana, India.

L. Shiva Shankar Reddy,
Department of Pharmaceutical Analysis, Santhiram College of Pharmacy, Nandyal-518501, Andhra Pradesh, India.

D. Madhuri,
Department of Pharmaceutical Analysis, Creative Education Society’s College of Pharmacy, NH-7, Chinnetekur, Kurnool-518218, A. P. India.

N. Yellasubbaiah,
Department of Pharmaceutical Analysis, Santhiram College of Pharmacy, Nandyal-518501, Andhra Pradesh, India.

S. V. Suresh Kumar,
Department of Pharmaceutical Analysis, Santhiram College of Pharmacy, Nandyal-518501, Andhra Pradesh, India.

N. Madana Gopal,
Department of Pharmaceutical Analysis, Santhiram College of Pharmacy, Nandyal-518501, Andhra Pradesh, India.

S. Indradev,
Department of Pharmaceutical Analysis, Creative Education Society’s College of Pharmacy, NH-7, Chinnetekur, Kurnool-518218, A. P. India.

S. Vinay,
Department of Pharmaceutical Analysis, Creative Education Society’s College of Pharmacy, NH-7, Chinnetekur, Kurnool-518218, A. P. India.

S. Mahesh,
Department of Pharmaceutical Analysis, Creative Education Society’s College of Pharmacy, NH-7, Chinnetekur, Kurnool-518218, A. P. India.

P. Ajay Kumar,
Department of Pharmaceutical Analysis, Creative Education Society’s College of Pharmacy, NH-7, Chinnetekur, Kurnool-518218, A. P. India.

Please see the link here: https://stm.bookpi.org/NAPR-V1/article/view/10426

UV and RP-HPLC Method Development and Validation for Estimation of Cilnidipine| Chapter 7 | Current Overview on Pharmaceutical Science Vol. 3

 The current experiment was devised to develop and endorse a simple RP-HPLC methods for determining Cilnidipine. Cilnidipine was quantified utilizing UV spectroscopy. Action separation of Cilnidipine was realized by using a C18 pillar. Mobile phase blend of methanol: water (90:10 v/v) was pressure at the flow of 1 ml/min. Detection At 241 nm, detection was completed activity. In accordance with the International Conference on Harmonization's (ICH) Q2R1 recommendations, confirmation parameters were determined. With a parametric value (r2) of 0.999, the uniformity curve for cilnidipine was linear over the range of 2–10 mg/ml.  The plan was noticed to be correct (101.66% recovery), exact (intraday, 1.65 and inter epoch, 1.38) and robust (% RSD was premeditated to be 0.66, 0.58 and 0.81 for alternative in mobile phase arrangement, wave length and flow speed respectively) for the reasoning of Cilnidipine. The developed system has successfully achieved all validation tests and can be used to estimate the attendance of cilnidipine in both drug formulations and bulk.

Author(s) Details:

Biswajit Samantaray,
School of Pharmacy and Life Sciences, Centurion University of Technology and Management, Odisha, India.

Satish Kanhar,
School of Pharmacy and Life Sciences, Centurion University of Technology and Management, Odisha, India.

Ashirbad Nanda,
School of Pharmacy and Life Sciences, Centurion University of Technology and Management, Odisha, India.

Please see the link here: https://stm.bookpi.org/COPS-V3/article/view/9112

Development and Validation of UV-Spectrophotometric Method for the Determination of Lornoxicam in Microsponges | Chapter 11 | Technological Innovation in Pharmaceutical Research Vol. 4

 Objective: To develop and verify a new UV-spectrophotometric method for estimating the amount of lornoxicam in microsponges that is specific, precise, and simple. Lornoxicam is an oxicam-class non-steroidal anti-inflammatory medication (NSAID) with analgesic, anti-inflammatory, and antipyretic (fever-reducing) effects.

Thermo Scientific Evolution 201 UV-Vis spectrophotometer was used to make UV-spectrophotometric measurements with methanol as the medium. The absorption maximum (max) of the standard solution was determined by running its spectra from 200 to 400 nm. Lornoxicam has a maximum wavelength of 353 nm. The absorbance of standard solutions containing 3, 6, 9, 12, and 15 g/ml of drug solution was measured against a blank at an absorption maximum of 353 nm. The concentration was then plotted on the X-axis and the absorbance on the Y-axis, yielding a straight line. As per ICH recommendations, validation criteria such as linearity and range, selectivity and specificity, LOD and LOQ, accuracy, precision, and robustness were assessed.

With a correlation coefficient of 0.9995, the UV-spectrophotometric technique was found to be linear over a concentration range of 3.0-15.0 g/ml. Lornoxicam has a limit of detection (LOD) of 1.26 g/ml and a limit of quantification (LOQ) of 3.82 g/ml, respectively. The accuracy ranged from 99.21 to 99.60 percent. The percent RSD for repeatability, intraday precision, and interday precision was determined to be 0.473, 0.478-0.619, and 0.855-1.818, respectively. The UV technique proposed is shown to be reliable.

Conclusion: The suggested UV-Visible spectrophotometric method was verified using ICH recommendations, and the findings and statistical parameters showed that the developed method is sensitive, precise, reliable, and easy for estimating the amount of lornoxicam in microsponges.

Author (s) Details

Ayya Rajendra Prasad
Department of Pharmaceutics, Nirmala College of Pharmacy, Mangalagiri 522503, Andhra Pradesh, India.

Bannaravuri Thireesha
Department of Pharmaceutics, Nirmala College of Pharmacy, Mangalagiri 522503, Andhra Pradesh, India.

View Book :- https://stm.bookpi.org/TIPR-V4/article/view/1503