Background: A good number of herbal drugs are known to possess immunomodulatory properties and generally act by stimulating both specific and nonspecific immunity. The plants, Andrographis paniculata (Kalmegh) and Tinospora cordifolia (Giloya), are becoming increasingly popular for a variety of diseases and infectious conditions, primarily influencing the host defence mechanism.
Objective: This study mainly focuses on the immunomodulatory
effect of different drug combinations (DC-I to DC-V) of methanolic extracts of
aerial parts of Andrographis
paniculata (AP) and mature stems of Tinospora cordifolia (TC).
Materials and Methods: The Aerial parts of Andrographis paniculata and
selected mature stems of the Tinospora
cordifolia were collected. The coarse powder (40-mesh) of shade-dried
aerial parts of AP and mature stems of TC (500g each) was subjected separately
to successive extraction with 1000 mL each of petroleum ether (60-80oC)
followed by methanol. The doses of DC-I to DC-V were selected and were administered
orally at doses of 200 mg/kg body weight to Albino Wistar rats and compared
with the control group and standard drug, i.e. cyclophosphamide (100mg/kg),
respectively. The animals were randomly selected, marked to permit individual
identification and kept in the cages for 7 days prior to dosing to allow for
acclimatisation to the laboratory conditions. Results obtained were
statistically analysed by using One-way ANOVA followed by Dunnett’s comparison
test.
Results: The time-dependent effect of different Drug Combinations
(DC-I to DC-V) on morphometric functional changes in mice (peritoneal
macrophages) was evaluated. Results suggest that although all five tested
combinations (DC-I to DC-V) prepared using different proportions of methanol
extracts of A. paniculata and T.
cordifolia (100:Zero, 75:25, 50:50, 25:75 and Zero:100) showed a
significant increase in macrophase count, neutrophil adhesion (NA),
haemagglutinating antibody (HA) titre and delayed type hypersensitivity (DTH)
response. In rats immunised with sheep RBC, DC-I to DC-V enhanced the humoral
antibody response to the antigen and significantly potentiated the cellular
immunity by facilitating the footpad thickness response to sheep RBC in
sensitised rats on chosen experimental models.
Conclusion: The values of macrophase, NA, HA, DTH and cellular
responses of combination DC-II at a 200 mg/kg body weight were statistically
significant as compared to other combinations. Combination DC-II exhibited the
best immunostimulant potential, indicating that it has promising
immunomodulatory activity, as proposed from a commercial point of view.
Immunostimulant therapy may be beneficial for patients under a variety of
settings that include prevention and treatment of various infectious diseases,
warranting further investigation in future studies.
Author
(s) Details
Yadav R
Department of Pharmacy, MAJU, Rampur, U.P., India.
Yadav N
SRMS, College of Engg. and Tech., (Pharmacy), Bareilly, U.P.,
India.
Kharya
M D
Department of Pharmaceutical Sciences, Dr. H. S. Gour Central
University, Sagar, M.P., India
Please see the book here:- https://doi.org/10.9734/bpi/psnid/v6/5395
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